Polyethyleneimine-Modified Superparamagnetic Fe3o4 Nanoparticles for Lipase Immobilization: Characterization and Application

Polyethyleneimine-Modified Superparamagnetic Fe3o4 Nanoparticles for Lipase Immobilization: Characterization and Application Publisher

Khoobi M¹ ; Motevalizadeh SF¹ ; Asadgol Z² ; Forootanfar H³ ; Shafiee A¹ ; Faramarzi MA²

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1. Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, 1417614411, Iran
2. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran, 1417614411, Iran
3. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran

Source: Materials Chemistry and Physics Published:2015

Abstract

Magnetically separable nanospheres consisting of polyethyleneimine (PEI) and succinated PEI grafted on silica coated magnetite (Fe3O4) were prepared and characterized using Fourier transform infrared spectroscopy, thermogravimetric analysis, X-ray diffraction, vibrating sample magnetometer, scanning electron microscopy and transmission electron microscopy. The prepared magnetic nanoparticles were then applied for physical adsorption or covalent attachment of Thermomyces lanuginosa lipase (TLL) via glutaraldehyde or hexamethylene diisocyanate. The reusability, storage, pH and thermal stabilities of the immobilized enzymes compared to that of free lipase were examined. The obtained results showed that the immobilized lipase on MNPs@PEI-GLU was the best biocatalyst which retained 80% of its initial activity after 12 cycles of application. The immobilized lipase on the selected support (MNPs@PEI-GLU) was also applied for the synthesis of ethyl valerate. Following 24 h incubation of the immobilized lipase on the selected support in n-hexane and solvent free media, the esterification percentages were 72.9% and 28.9%, respectively. © 2014 Elsevier B.V. All rights reserved.

Style	Citing Format
MLA	Khoobi M, et al.. "Polyethyleneimine-Modified Superparamagnetic Fe3o4 Nanoparticles for Lipase Immobilization: Characterization and Application." Materials Chemistry and Physics, vol. 149, no. , 2015, pp. 77-86.
APA	Khoobi M, Motevalizadeh SF, Asadgol Z, Forootanfar H, Shafiee A, Faramarzi MA (2015). Polyethyleneimine-Modified Superparamagnetic Fe3o4 Nanoparticles for Lipase Immobilization: Characterization and Application. Materials Chemistry and Physics, 149(), 77-86.
Chicago	Khoobi M, Motevalizadeh SF, Asadgol Z, Forootanfar H, Shafiee A, Faramarzi MA. "Polyethyleneimine-Modified Superparamagnetic Fe3o4 Nanoparticles for Lipase Immobilization: Characterization and Application." Materials Chemistry and Physics 149, no. (2015): 77-86.
Harvard	Khoobi M et al. (2015) 'Polyethyleneimine-Modified Superparamagnetic Fe3o4 Nanoparticles for Lipase Immobilization: Characterization and Application', Materials Chemistry and Physics, 149(), pp. 77-86.
Vancouver	Khoobi M, Motevalizadeh SF, Asadgol Z, Forootanfar H, Shafiee A, Faramarzi MA. Polyethyleneimine-Modified Superparamagnetic Fe3o4 Nanoparticles for Lipase Immobilization: Characterization and Application. Materials Chemistry and Physics. 2015;149():77-86.
BibTex	@article{ author = {Khoobi M and Motevalizadeh SF and Asadgol Z and Forootanfar H and Shafiee A and Faramarzi MA}, title = {Polyethyleneimine-Modified Superparamagnetic Fe3o4 Nanoparticles for Lipase Immobilization: Characterization and Application}, journal = {Materials Chemistry and Physics}, volume = {149}, number = {}, pages = {77-86}, year = {2015} }
RIS	TY - JOUR AU - Khoobi M AU - Motevalizadeh SF AU - Asadgol Z AU - Forootanfar H AU - Shafiee A AU - Faramarzi MA TI - Polyethyleneimine-Modified Superparamagnetic Fe3o4 Nanoparticles for Lipase Immobilization: Characterization and Application JO - Materials Chemistry and Physics VL - 149 IS - SP - 77 EP - 86 PY - 2015 ER -

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