Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Antidepressive Synergism Between Crocin and D-Ap5 in Acute Restraint-Stressed Mice Publisher Pubmed



Gerami SS1 ; Ebrahimighiri M2 ; Khakpai F3 ; Zarrindast MR4, 5, 6
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Pharmacology, Faculty of Pharmacy, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
  2. 2. Department of Biology, Faculty of Sciences, University of Zanjan, Zanjan, Iran
  3. 3. Department of Physiology, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Iran
  4. 4. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Iran
  5. 5. Iranian National Center for Addiction Studies, Iran
  6. 6. Department of Cognitive Neuroscience, Institute for Cognitive Science Studies (ICSS), Tehran, Iran

Source: Behavioural Pharmacology Published:2024


Abstract

Emerging evidence suggests that crocin rescues stress-induced depressive symptoms in mice via stimulation of hippocampal neurogenesis. Glutamate modulators mainly involving N-methyl-d-aspartate (NMDA) receptors (NMDARs) have highlighted a role in neural development, synaptic plasticity, and depression. The research presented here was designed to appraise the interaction between NMDAR agents and crocin on depressive-related behaviors in the NMRI male mice exposed to acute restraint stress (ARS) for a period of 4 h. The mice were submitted to the splash test, forced swimming test, and tail suspension test to evaluate depressive-like behavior. The ARS decreased the grooming duration in the splash test and increased immobility time in the forced swimming test and tail suspension test, suggesting a depressive-like phenotype. NMDA (0.25 and 0.5 μg/mouse, intracerebroventricular) did not alter depression-related profiles in both non-acute restraint stress (NARS) and ARS mice, while the same doses of NMDAR antagonist D-AP5 potentiated the antidepressive-like activities in the ARS mice compared with the NARS mice. Moreover, a low dose of NMDA did not change depression-related parameters in the crocin-treated NARS or ARS mice, while D-AP5 enhanced the crocin response in the NARS and ARS mice. Isobologram analysis noted a synergism between crocin and D-AP5 on antidepressive-like behavior in the NARS and ARS mice. Collectively, the combination of crocin and D-AP5 was shown to mitigate depression symptoms and can be potentially used for the treatment of depression disorders. Copyright © 2024 Wolters Kluwer Health, Inc. All rights reserved.
Experts (# of related papers)