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Urinary Exosomal Expression of Long Non-Coding Rnas As Diagnostic Marker in Bladder Cancer Publisher



Yazarlou F1 ; Modarressi MH1 ; Mowla SJ2 ; Oskooei VK3 ; Motevaseli E4 ; Tooli LF5 ; Nekoohesh L6 ; Eghbali M1 ; Ghafourifard S3 ; Afsharpad M7
Authors
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Authors Affiliations
  1. 1. Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Faculty of Biological Sciences, Department of Genetics, Tarbiat Modares University, Tehran, Iran
  3. 3. Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  4. 4. Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Microbiology, School of Biology, College of Science, Tehran University, Tehran, Iran
  6. 6. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  7. 7. Cancer Control Research Center, Cancer Control Foundation, Iran University of Medical Sciences, Tehran, Iran

Source: Cancer Management and Research Published:2018


Abstract

Background: Long non-coding RNAs (lncRNAs) and exosomes have been regarded as components of cell signal transmission that modulate indigenous cellular microenvironments. Exosomes also participate in relocation of functional lncRNAs between cells. Methods: In the present study, we evaluated expression of LINC00355, LINC00958, UCA1-201, UCA1-203, and MALAT1 lncRNAs in urinary exosomes isolated from transitional cell carcinoma (TCC) of bladder, non-malignant urinary disorders, and normal subjects. Results: LINC00355, UCA1-203, and MALAT1 expression was significantly higher in TCC patients compared to controls (non-malignant or normal samples). However, UCA1-201 expression was significantly decreased in TCC patients compared with controls. LINC00355 and MALAT1 expression was significantly lower in cigarette smokers and opium-addicted TCC patients, respectively. On the other hand, LINC00355 expression tended to be higher in opium-addicted TCC patients. The proposed panel of lncRNAs (composed of UCA1-201, UCA1-203, MALAT1, and LINC00355) had 92% sensitivity and 91.7% specificity for diagnosis of bladder cancer from normal samples. Conclusion: Transcript levels of lncRNAs in urinary exosomes are potential diagnostic biomarkers in bladder cancer. © 2018 Yazarlou et al.
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