Olfactory Epithelium As an Infinitive Source of Neural Stem Cells for Derivation of Inner Ear Hair Cells; [El Epitelio Olfatorio Como Fuente Infinita De Celulas Madre Neurales Para La Derivacion De Los Vestibulocitos Del Oido Interno]

Olfactory Epithelium As an Infinitive Source of Neural Stem Cells for Derivation of Inner Ear Hair Cells; [El Epitelio Olfatorio Como Fuente Infinita De Celulas Madre Neurales Para La Derivacion De Los Vestibulocitos Del Oido Interno] Publisher

Bahmani T¹ ; Gholami S¹ ; Mostafaie A² ; Karimi E³ ; Hoseinkhani Z¹ ; Azad T⁴ ; Mahdizadeh H⁶ ; Mansouri K¹

Show Affiliations

1. Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
2. Department of Immunology, Kermanshah University of Medical Sciences, Kermanshah, Iran
3. Department of Otorhinolaryngology, Tehran University of Medical Sciences, Tehran, Iran
4. Department of Biochemistry, Tarbiat Modarres University, Tehran, Iran
5. Babol University of Medical Sciences, Babol, Iran
6. Cellular and Molecular Research Medicine, Babol University of Medical Sciences, Babol, Iran

Source: International Journal of Morphology Published:2017

Abstract

Surgical techniques for treatment of sensory neural hearing loss (SNHL) have unpredictable outcomes and in recent years cell therapy investigated for treatment of SNHL. Olfactory epithelium proceed neurogenesis during life time and provide an easily accessible source of neural stem cells. So the aim of this study was isolating neural stem cells from olfactory epithelium of rat and differentiation of these cells into hair cells of inner ear in vitro. The epithelium tissue of olfactory mucosa of rats were removed and digested by collagenase H. The digested tissue was cultured in flasks in suspension forms to create spheres. Spheres were passaged and from passage 2 spheres selected for differentiation. At this stage cells of spheres isolated from each other and placed in flask containing defined differentiation medium. Cells at this stage cultured in adhesive form. Immunohistochemistry and RT-PCR were used for neural stem cells and hair cells identification. Spheres formed from olfactory epithelium culture and immunohistochemistry revealed that cells of spheres from passage one and two expressed the neural stem cells markers. After culture of isolated cells in differentiation medium, the morphology of cells begun to change. The cells presented neural cells projections and after 10 days the projections elongated more and interact to each other in multi layers. RT-PCR and immunohistochemistry revealed that differentiated cells expressed hair cells specific genes. In this study we showed that neural stem cells of olfactory epithelium can differentiate into hair cells of inner ear and therefore can be used for treatment of SNHL. © 2017, Universidad de la Frontera. All rights reserved.

Style	Citing Format
MLA	Bahmani T, et al.. "Olfactory Epithelium As an Infinitive Source of Neural Stem Cells for Derivation of Inner Ear Hair Cells; [El Epitelio Olfatorio Como Fuente Infinita De Celulas Madre Neurales Para La Derivacion De Los Vestibulocitos Del Oido Interno]." International Journal of Morphology, vol. 35, no. 2, 2017, pp. 413-419.
APA	Bahmani T, Gholami S, Mostafaie A, Karimi E, Hoseinkhani Z, Azad T, Mahdizadeh H, Mansouri K (2017). Olfactory Epithelium As an Infinitive Source of Neural Stem Cells for Derivation of Inner Ear Hair Cells; [El Epitelio Olfatorio Como Fuente Infinita De Celulas Madre Neurales Para La Derivacion De Los Vestibulocitos Del Oido Interno]. International Journal of Morphology, 35(2), 413-419.
Chicago	Bahmani T, Gholami S, Mostafaie A, Karimi E, Hoseinkhani Z, Azad T, Mahdizadeh H, Mansouri K. "Olfactory Epithelium As an Infinitive Source of Neural Stem Cells for Derivation of Inner Ear Hair Cells; [El Epitelio Olfatorio Como Fuente Infinita De Celulas Madre Neurales Para La Derivacion De Los Vestibulocitos Del Oido Interno]." International Journal of Morphology 35, no. 2 (2017): 413-419.
Harvard	Bahmani T et al. (2017) 'Olfactory Epithelium As an Infinitive Source of Neural Stem Cells for Derivation of Inner Ear Hair Cells; [El Epitelio Olfatorio Como Fuente Infinita De Celulas Madre Neurales Para La Derivacion De Los Vestibulocitos Del Oido Interno]', International Journal of Morphology, 35(2), pp. 413-419.
Vancouver	Bahmani T, Gholami S, Mostafaie A, Karimi E, Hoseinkhani Z, Azad T, et al.. Olfactory Epithelium As an Infinitive Source of Neural Stem Cells for Derivation of Inner Ear Hair Cells; [El Epitelio Olfatorio Como Fuente Infinita De Celulas Madre Neurales Para La Derivacion De Los Vestibulocitos Del Oido Interno]. International Journal of Morphology. 2017;35(2):413-419.
BibTex	@article{ author = {Bahmani T and Gholami S and Mostafaie A and Karimi E and Hoseinkhani Z and Azad T and Mahdizadeh H and Mansouri K}, title = {Olfactory Epithelium As an Infinitive Source of Neural Stem Cells for Derivation of Inner Ear Hair Cells; [El Epitelio Olfatorio Como Fuente Infinita De Celulas Madre Neurales Para La Derivacion De Los Vestibulocitos Del Oido Interno]}, journal = {International Journal of Morphology}, volume = {35}, number = {2}, pages = {413-419}, year = {2017} }
RIS	TY - JOUR AU - Bahmani T AU - Gholami S AU - Mostafaie A AU - Karimi E AU - Hoseinkhani Z AU - Azad T AU - Mahdizadeh H AU - Mansouri K TI - Olfactory Epithelium As an Infinitive Source of Neural Stem Cells for Derivation of Inner Ear Hair Cells; [El Epitelio Olfatorio Como Fuente Infinita De Celulas Madre Neurales Para La Derivacion De Los Vestibulocitos Del Oido Interno] JO - International Journal of Morphology VL - 35 IS - 2 SP - 413 EP - 419 PY - 2017 ER -

Style

Citing Format

MLA

Bahmani T, et al.. "Olfactory Epithelium As an Infinitive Source of Neural Stem Cells for Derivation of Inner Ear Hair Cells; [El Epitelio Olfatorio Como Fuente Infinita De Celulas Madre Neurales Para La Derivacion De Los Vestibulocitos Del Oido Interno]." International Journal of Morphology, vol. 35, no. 2, 2017, pp. 413-419.

APA

Bahmani T, Gholami S, Mostafaie A, Karimi E, Hoseinkhani Z, Azad T, Mahdizadeh H, Mansouri K (2017). Olfactory Epithelium As an Infinitive Source of Neural Stem Cells for Derivation of Inner Ear Hair Cells; [El Epitelio Olfatorio Como Fuente Infinita De Celulas Madre Neurales Para La Derivacion De Los Vestibulocitos Del Oido Interno]. International Journal of Morphology, 35(2), 413-419.

Chicago

Bahmani T, Gholami S, Mostafaie A, Karimi E, Hoseinkhani Z, Azad T, Mahdizadeh H, Mansouri K. "Olfactory Epithelium As an Infinitive Source of Neural Stem Cells for Derivation of Inner Ear Hair Cells; [El Epitelio Olfatorio Como Fuente Infinita De Celulas Madre Neurales Para La Derivacion De Los Vestibulocitos Del Oido Interno]." International Journal of Morphology 35, no. 2 (2017): 413-419.

Harvard

Bahmani T et al. (2017) 'Olfactory Epithelium As an Infinitive Source of Neural Stem Cells for Derivation of Inner Ear Hair Cells; [El Epitelio Olfatorio Como Fuente Infinita De Celulas Madre Neurales Para La Derivacion De Los Vestibulocitos Del Oido Interno]', International Journal of Morphology, 35(2), pp. 413-419.

Vancouver

Bahmani T, Gholami S, Mostafaie A, Karimi E, Hoseinkhani Z, Azad T, et al.. Olfactory Epithelium As an Infinitive Source of Neural Stem Cells for Derivation of Inner Ear Hair Cells; [El Epitelio Olfatorio Como Fuente Infinita De Celulas Madre Neurales Para La Derivacion De Los Vestibulocitos Del Oido Interno]. International Journal of Morphology. 2017;35(2):413-419.

BibTex

@article{ author = {Bahmani T and Gholami S and Mostafaie A and Karimi E and Hoseinkhani Z and Azad T and Mahdizadeh H and Mansouri K}, title = {Olfactory Epithelium As an Infinitive Source of Neural Stem Cells for Derivation of Inner Ear Hair Cells; [El Epitelio Olfatorio Como Fuente Infinita De Celulas Madre Neurales Para La Derivacion De Los Vestibulocitos Del Oido Interno]}, journal = {International Journal of Morphology}, volume = {35}, number = {2}, pages = {413-419}, year = {2017} }

RIS

TY - JOUR
AU - Bahmani T
AU - Gholami S
AU - Mostafaie A
AU - Karimi E
AU - Hoseinkhani Z
AU - Azad T
AU - Mahdizadeh H
AU - Mansouri K
TI - Olfactory Epithelium As an Infinitive Source of Neural Stem Cells for Derivation of Inner Ear Hair Cells; [El Epitelio Olfatorio Como Fuente Infinita De Celulas Madre Neurales Para La Derivacion De Los Vestibulocitos Del Oido Interno]
JO - International Journal of Morphology
VL - 35
IS - 2
SP - 413
EP - 419
PY - 2017
ER -

Science Communicator Platform

Authors

Authors Affiliations

Abstract