The Therapeutic Potential of Human Adipose-Derived Mesenchymal Stem Cells Producing Cxcl10 in a Mouse Melanoma Lung Metastasis Model

The Therapeutic Potential of Human Adipose-Derived Mesenchymal Stem Cells Producing Cxcl10 in a Mouse Melanoma Lung Metastasis Model Publisher Pubmed

Mirzaei H¹ ; Salehi H² ; Oskuee RK¹ ; Mohammadpour A³ ; Mirzaei HR^{4, 5} ; Sharifi MR⁶ ; Salarinia R⁷ ; Darani HY⁸ ; Mokhtari M⁹ ; Masoudifar A¹⁰ ; Sahebkar A^{11, 12} ; Salehi R⁶ ; Jaafari MR^{13, 14}

Source: Cancer Letters Published:2018

Abstract

Interferon γ-induced protein 10 kDa (IP-10) is a potent chemoattractant and has been suggested to enhance antitumor activity and mediate tumor regression through multiple mechanisms of action. Multiple lines of evidence have indicated that genetically-modified adult stem cells represent a potential source for cell-based cancer therapy. In the current study, we assessed therapeutic potential of human adipose derived mesenchymal stem cells (hADSC) genetically-modified to express IP-10 for the treatment of lung metastasis in an immunocompetent mouse model of metastatic melanoma. A Piggybac vector encoding IP-10 was employed to transfect hADSC ex vivo. Expression and bioactivity of the transgenic protein from hADSCs expressing IP-10 were confirmed prior to in vivo studies. Our results indicated that hADSCs expressing IP-10 could inhibit the growth of B16F10 melanoma cells and significantly prolonged survival. Immunohistochemistry analysis, TUNEL assay and western blot analysis indicated that hADSCs expressing IP-10 inhibited tumor cell growth, hindered tumor infiltration of Tregs, restricted angiogenesis and significantly prolonged survival. In conclusion, our results demonstrated that targeting metastatic tumor sites by hADSC expressing IP-10 could reduce melanoma tumor growth and lung metastasis. © 2018 Elsevier B.V.

Style	Citing Format
MLA	Mirzaei H, et al.. "The Therapeutic Potential of Human Adipose-Derived Mesenchymal Stem Cells Producing Cxcl10 in a Mouse Melanoma Lung Metastasis Model." Cancer Letters, vol. 419, no. , 2018, pp. 30-39.
APA	Mirzaei H, Salehi H, Oskuee RK, Mohammadpour A, Mirzaei HR, Sharifi MR, Salarinia R, Darani HY, Mokhtari M, Masoudifar A, Sahebkar A, Salehi R, Jaafari MR (2018). The Therapeutic Potential of Human Adipose-Derived Mesenchymal Stem Cells Producing Cxcl10 in a Mouse Melanoma Lung Metastasis Model. Cancer Letters, 419(), 30-39.
Chicago	Mirzaei H, Salehi H, Oskuee RK, Mohammadpour A, Mirzaei HR, Sharifi MR, Salarinia R, et al.. "The Therapeutic Potential of Human Adipose-Derived Mesenchymal Stem Cells Producing Cxcl10 in a Mouse Melanoma Lung Metastasis Model." Cancer Letters 419, no. (2018): 30-39.
Harvard	Mirzaei H et al. (2018) 'The Therapeutic Potential of Human Adipose-Derived Mesenchymal Stem Cells Producing Cxcl10 in a Mouse Melanoma Lung Metastasis Model', Cancer Letters, 419(), pp. 30-39.
Vancouver	Mirzaei H, Salehi H, Oskuee RK, Mohammadpour A, Mirzaei HR, Sharifi MR, et al.. The Therapeutic Potential of Human Adipose-Derived Mesenchymal Stem Cells Producing Cxcl10 in a Mouse Melanoma Lung Metastasis Model. Cancer Letters. 2018;419():30-39.
BibTex	@article{ author = {Mirzaei H and Salehi H and Oskuee RK and Mohammadpour A and Mirzaei HR and Sharifi MR and Salarinia R and Darani HY and Mokhtari M and Masoudifar A and Sahebkar A and Salehi R and Jaafari MR}, title = {The Therapeutic Potential of Human Adipose-Derived Mesenchymal Stem Cells Producing Cxcl10 in a Mouse Melanoma Lung Metastasis Model}, journal = {Cancer Letters}, volume = {419}, number = {}, pages = {30-39}, year = {2018} }
RIS	TY - JOUR AU - Mirzaei H AU - Salehi H AU - Oskuee RK AU - Mohammadpour A AU - Mirzaei HR AU - Sharifi MR AU - Salarinia R AU - Darani HY AU - Mokhtari M AU - Masoudifar A AU - Sahebkar A AU - Salehi R AU - Jaafari MR TI - The Therapeutic Potential of Human Adipose-Derived Mesenchymal Stem Cells Producing Cxcl10 in a Mouse Melanoma Lung Metastasis Model JO - Cancer Letters VL - 419 IS - SP - 30 EP - 39 PY - 2018 ER -

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