The Use of Camel Antibodies in Development of Egfrviii Enzyme-Linked Immunosorbent Assay

The Use of Camel Antibodies in Development of Egfrviii Enzyme-Linked Immunosorbent Assay Publisher

Sohi AN¹ ; Rajabibazl M^{2, 3} ; Rasaee MJ¹ ; Omidfar K⁴

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1. Department of Clinical Biochemistry, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2. Department of Clinical Biochemistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3. School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4. Endocrinology and Metabolism Research Center, Tehran University of Medical Sciences, Tehran, Iran

Source: Applied Biochemistry and Microbiology Published:2015

Abstract

In the present study, anti-EGFRvIII antibodies were purified from the serum of an immunized Arabian camel (Camelus dromedarius). The three subclasses of IgG were purified using protein-G column chromatography. Epidermal growth factor receptor vIII (EGFRvIII) was purified from HC220d2/c cell line expressed the highest levels of EGF receptors using a concanavalin A column chromatography. The ELISA procedure was developed using 2 types of enzyme labels namely horse radish peroxidase (HRP) and penicillinase. The affinity constants of purified total IgG and Ig fractions (IgG1, IgG2 and IgG3) calculated were found to be 4.48 × 107, 1.3 × 107, 4.23 × 106 and 1.67 × 106 M−1, respectively. The sensitivity of assays for the detection of EGFR when used total IgG were found to be 0.35 μg/mL in HRP label assay and 0.45 μg/mL in penicillinase label assay. We concluded that the ELISA in competitive or inhibition manners using polyclonal camel antibodies containing total IgG (but not IgG1, IgG2, and IgG3 fractions) can be promising in detection of EGFRvIII in biological fluids. © 2015, Pleiades Publishing, Inc.

Style	Citing Format
MLA	Sohi AN, et al.. "The Use of Camel Antibodies in Development of Egfrviii Enzyme-Linked Immunosorbent Assay." Applied Biochemistry and Microbiology, vol. 51, no. 3, 2015, pp. 374-380.
APA	Sohi AN, Rajabibazl M, Rasaee MJ, Omidfar K (2015). The Use of Camel Antibodies in Development of Egfrviii Enzyme-Linked Immunosorbent Assay. Applied Biochemistry and Microbiology, 51(3), 374-380.
Chicago	Sohi AN, Rajabibazl M, Rasaee MJ, Omidfar K. "The Use of Camel Antibodies in Development of Egfrviii Enzyme-Linked Immunosorbent Assay." Applied Biochemistry and Microbiology 51, no. 3 (2015): 374-380.
Harvard	Sohi AN et al. (2015) 'The Use of Camel Antibodies in Development of Egfrviii Enzyme-Linked Immunosorbent Assay', Applied Biochemistry and Microbiology, 51(3), pp. 374-380.
Vancouver	Sohi AN, Rajabibazl M, Rasaee MJ, Omidfar K. The Use of Camel Antibodies in Development of Egfrviii Enzyme-Linked Immunosorbent Assay. Applied Biochemistry and Microbiology. 2015;51(3):374-380.
BibTex	@article{ author = {Sohi AN and Rajabibazl M and Rasaee MJ and Omidfar K}, title = {The Use of Camel Antibodies in Development of Egfrviii Enzyme-Linked Immunosorbent Assay}, journal = {Applied Biochemistry and Microbiology}, volume = {51}, number = {3}, pages = {374-380}, year = {2015} }
RIS	TY - JOUR AU - Sohi AN AU - Rajabibazl M AU - Rasaee MJ AU - Omidfar K TI - The Use of Camel Antibodies in Development of Egfrviii Enzyme-Linked Immunosorbent Assay JO - Applied Biochemistry and Microbiology VL - 51 IS - 3 SP - 374 EP - 380 PY - 2015 ER -

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