Correlation Between Qace and Qaceδ1 Efflux Pump Genes, Antibiotic and Disinfectant Resistant Among Clinical Isolates of E.Coli Publisher Pubmed



Shafaati M¹ ; Boroumand M² ; Nowroozi J³ ; Amiri P⁴ ; Kazemian H^{5, 6} Show Affiliations
Source: Recent Patents on Anti-Infective Drug Discovery Published:2016

Abstract

Introduction: Antiseptics and disinfectants have been used widely in hospitals and other health care settings to control the growth of microorganisms. However, some disinfectant resistant strains were reported. The objectives of our study were to evaluate correlation between the efflux pump genes, drugs and disinfectant resistant among clinical isolates of E.coli. Methods: A total of 102 of E. coli strains were isolated from urine sample of hospitalized patients. The antibiotic susceptibility was carried out by disc diffusion method. Didecyl di-methyl ammonium chloride (DDDMAC) was used as Quaternary ammonium compound (QAC) disinfectant which was used in Heart Center Hospital. PCR reaction was carried out for detection of qacE and qacΔE efflux pump genes. Result: Almost all the strains had higher resistance to ampicillin, ciproflaxacin, cotrimaxazole and cephalothin. Totally 49% (n: 50) of strains were produced ESBL. Almost all the strains have MIC value between 0.00195 to 0.0078 mg/l for DDDMAC. Correlation between presence of qacE and qacΔE genes and antibiotic resistance was perceived. Presence of qacE and qacΔE genes among strains that have high disinfectant MIC value were 96.9% and 93.7% respectively. In addition, 98% of ESBL producing strains harbored qacE gene and 94% of ESBL producing strains harbored qacΔE gene. Conclusion: Our study indicated that there was a strong correlation between presence of qacE and qacΔE genes with resistance to some antibiotics and growth in media which contain high concentration of disinfectant. In conclusion, other mechanisms also play important role in resistant to antimicrobial agents but the role of efflux pumps in resistant to antimicrobial agents should not be neglected. © 2016 Bentham Science Publishers.