Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Dapsone Reduced Cuprizone-Induced Demyelination Via Targeting Nrf2 and Ikb in C57bl/6 Mice Publisher



Dehpour AR1, 2 ; Khaledi E3 ; Noori T4 ; Mohammadifarani A5, 6 ; Delphi L7 ; Sureda A8, 9 ; Sobarzosanchez E10, 11 ; Shirooie S4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Experimental Medicine Research Center, Tehran University of medical sciences, Tehran, Iran
  3. 3. Student Research Committee, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran
  4. 4. Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
  5. 5. Medical Plant Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
  6. 6. Department of Physiology and Pharmacology, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran
  7. 7. Animal Biology Department, Faculty of Biology, College of Sciences, University of Tehran, Tehran, Iran
  8. 8. Research Group on Community Nutrition and Oxidative Stress (NUCOX), Health Research Institute of Balearic Islands (IdISBa), University of Balearic Islands, Palma de Mallorca, E-07122, Spain
  9. 9. CIBER Fisiopatologia de la Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III (ISCIII), Madrid, 28029, Spain
  10. 10. Instituto de Investigacion y Postgrado, Facultad de Ciencias de la Salud, Universidad Central de Chile, Chile
  11. 11. Department of Organic Chemistry, Faculty of Pharmacy, University of Santiago de Compostela, Spain

Source: Iranian Journal of Basic Medical Sciences Published:2022


Abstract

Objective(s): Multiple Sclerosis (MS) is an inflammatory disorder wherein the myelin of nerve cells in the central nervous system is damaged. In the current study, we assessed the effect of Dapsone (DAP) on the improvement of behavioral dysfunction and preservation of myelin in the cuprizone (CPZ) induced demyelination model via targeting Nrf2 and IKB. Materials and Methods: MS was induced in C57BL/6 mice through diet supplementation of CPZ (0.2%) for 6 weeks, and DAP (12.5 mg/kg/day; IP) was administered for the last 2 weeks of treatment. Pole test and rotarod performance test, LFB and H&E staining, and Immunohistochemistry (IHC) staining of p-Nrf2 and p-IKB were performed. Furthermore, superoxide dismutase (SOD) and nitrite were measured. Results: DAP treatment prevented body loss induced by CPZ (P<0.001). Pole test showed that CPZ increased latency time to fall (P<0.0001) but the latency to reach the floor in the DAP-CPZ group was significantly shorter (P<0.0001). Rotarod performance test showed the effect of CPZ in reducing fall time in the CPZ group (P<0.0014); however, DAP significantly increased fall time (P=0.0012). In LFB staining, DAP reduced demyelination induced by CPZ. CPZ significantly decreased p-Nrf2 and elevated p-IKB levels compared with the control group (P<0.0001), but in DAP-treated groups markedly modified these changes (P<0.0001). CPZ increased the brain nitrite levels and reduced SOD activity, but in DAP-treated considerably reversed CPZ-induced changes. Conclusion: These data support the suggestion that the beneficial properties of DAP on the CPZ-induced demyelination are mediated by targeting Nrf2 and NF-kB pathways. © 2022 Mashhad University of Medical Sciences. All rights reserved.
Related Docs
View other Related Docs
1. Trifluoperazine Reduces Cuprizone-Induced Demyelination Via Targeting Nrf2 and Ikb in Mice, European Journal of Pharmacology (2021)
2. A Comprehensive Insight Into the Anti-Inflammatory Properties of Dapsone, Naunyn-Schmiedeberg's Archives of Pharmacology (2022)
3. Dapsone Ameliorates Colitis Through Tlr4/Nf-Kb Pathway in Tnbs Induced Colitis Model in Rat, Archives of Medical Research (2021)
4. Protective Effect of Dapsone Against Renal Ischemia-Reperfusion Injury in Rat, Immunopharmacology and Immunotoxicology (2020)
5. The Effect of Dapsone in Testosterone Enanthate-Induced Polycystic Ovary Syndrome in Rat, Journal of Steroid Biochemistry and Molecular Biology (2021)
6. Dapsone Improves the Vincristine-Induced Neuropathic Nociception by Modulating Neuroinflammation and Oxidative Stress, DARU# Journal of Pharmaceutical Sciences (2022)
7. Protective Effect of Dapsone Against Bleomycin-Induced Lung Fibrosis in Rat, Experimental and Molecular Pathology (2022)
8. Synthesis and Evaluation of the Effects of Solid Lipid Nanoparticles of Ivermectin and Ivermectin on Cuprizone-Induced Demyelination Via Targeting the Trpa1/Nf-Kb/Gfap Signaling Pathway, Iranian Journal of Basic Medical Sciences (2023)
Experts (# of related papers)
View all Related Experts
Ahmad Reza Dehpour (15)
Iraj Ragerdi Kashani (4)
Other Related Docs
9. Metformin Therapy Attenuates Pro-Inflammatory Microglia by Inhibiting Nf-Κb in Cuprizone Demyelinating Mouse Model of Multiple Sclerosis, Neurotoxicity Research (2021)
10. The Anti-Inflammatory Effect of Dapsone on Ovalbumin-Induced Allergic Rhinitis in Balb/C Mice, Life Sciences (2022)
11. Antibiotics With Therapeutic Effects on Spinal Cord Injury: A Review, Fundamental and Clinical Pharmacology (2021)
12. Mesenchymal Stem Cells Ameliorate Cuprizone-Induced Demyelination by Targeting Oxidative Stress and Mitochondrial Dysfunction, Cellular and Molecular Neurobiology (2021)
13. Coenzyme Q10 Enhances Remyelination and Regulate Inflammation Effects of Cuprizone in Corpus Callosum of Chronic Model of Multiple Sclerosis, Journal of Molecular Histology (2021)
14. Protective Effects of Erythropoietin Against Cuprizone-Induced Oxidative Stress and Demyelination in the Mouse Corpus Callosum, Iranian Journal of Basic Medical Sciences (2017)
15. The Therapeutic Activities of Metformin: Focus on the Nrf2 Signaling Pathway and Oxidative Stress Amelioration, Current Molecular Pharmacology (2023)
16. Sumatriptan Mitigates Bleomycin-Induced Lung Fibrosis in Male Rats: Involvement of Inflammation, Oxidative Stress and Α-Sma, Tissue and Cell (2024)
17. The Protective Effect of Dapsone Against Ethanol, Stress, and Indomethacin-Induced Gastric Erosion in Rats, Acta Medica Iranica (2021)
18. Beneficial Effects of Dapsone on Ischemia/Reperfusion Injury Following Torsion/Detorsion in Ipsilateral and Contralateral Testes in Rat, Theriogenology (2019)
19. Cardioprotective Effects of Dapsone Against Doxorubicin-Induced Cardiotoxicity in Rats, Cancer Chemotherapy and Pharmacology (2020)
20. Ivermectin Ameliorates Bleomycin-Induced Lung Fibrosis in Male Rats by Inhibiting the Inflammation and Oxidative Stress, Immunopharmacology and Immunotoxicology (2024)
21. Anti-Lingo -1 Improved Remyelination and Neurobehavioral Deficit in Cuprizone-Induced Demyelination, Iranian Journal of Basic Medical Sciences (2021)
22. Ameliorative Effects of Acetyl-L-Carnitine on Corpus Callosum and Functional Recovery in Demyelinated Mouse Model, International Journal of Neuroscience (2024)
23. Nrf2 Signaling Pathway: A Comprehensive Prognostic and Gene Expression Profile Analysis in Breast Cancer, Pathology Research and Practice (2023)
24. Efficacy of Disease Modifying Therapies in Progressive Ms and How Immune Senescence May Explain Their Failure, Frontiers in Neurology (2022)
25. Protective Effect of a Camp Analogue on Behavioral Deficits and Neuropathological Changes in Cuprizone Model of Demyelination, Molecular Neurobiology (2015)
26. Oligoprotective Effect of Metformin Through the Ampk-Dependent on Restoration of Mitochondrial Hemostasis in the Cuprizone-Induced Multiple Sclerosis Model, Journal of Molecular Histology (2019)