A Novel Clot Lysis Assay for Recombinant Plasminogen Activator

A Novel Clot Lysis Assay for Recombinant Plasminogen Activator Publisher Pubmed

Jamialahmadi O¹ ; Fazeli A² ; Hasheminajafabadi S¹ ; Fazeli MR³

Show Affiliations

1. Biotechnology Group, Tarbiat Modares University, P.O. Box: 14115-114, Tehran, Iran
2. Department of Research & Development, Zistdaru Danesh Company, Tehran, Iran
3. Department of Drug & Food Control and Pharmaceutical Quality Assurance Research Center, Tehran University of Medical Sciences, Tehran, Iran

Source: Biotechnology Letters Published:2015

Abstract

Recombinant plasminogen activator (r-PA, reteplase) is an engineered variant of alteplase. When expressed in E. coli, it appears as inclusion bodies that require refolding to recover its biological activity. An important step following refolding is to determine the activity of refolded protein. Current methods for enzymatic activity of thrombolytic drugs are costly and complex. Here a straightforward and low-cost clot lysis assay was developed. It quantitatively measures the activity of the commercial reteplase and is also capable of screening refolding conditions. As evidence for adequate accuracy and sensitivity of the current assay, r-PA activity measurements are shown to be comparable to those obtained from chromogenic substrate assay. © 2014, Springer Science+Business Media Dordrecht.

2. Scale up and Pharmacokinetic Study of a Novel Mutated Chimeric Tissue Plasminogen Activator (Mt-Pa) in Rats, Scientific Reports (2017)

3. A Simplified Process for Purification and Refolding of Recombinant Human Interferon-Α2b, Iranian Biomedical Journal (2022)

Experts (# of related papers)

Mohammad Reza Fazeli (2)

Mohammadreza Rouini (1)

Style	Citing Format
MLA	Jamialahmadi O, et al.. "A Novel Clot Lysis Assay for Recombinant Plasminogen Activator." Biotechnology Letters, vol. 37, no. 3, 2015, pp. 593-600.
APA	Jamialahmadi O, Fazeli A, Hasheminajafabadi S, Fazeli MR (2015). A Novel Clot Lysis Assay for Recombinant Plasminogen Activator. Biotechnology Letters, 37(3), 593-600.
Chicago	Jamialahmadi O, Fazeli A, Hasheminajafabadi S, Fazeli MR. "A Novel Clot Lysis Assay for Recombinant Plasminogen Activator." Biotechnology Letters 37, no. 3 (2015): 593-600.
Harvard	Jamialahmadi O et al. (2015) 'A Novel Clot Lysis Assay for Recombinant Plasminogen Activator', Biotechnology Letters, 37(3), pp. 593-600.
Vancouver	Jamialahmadi O, Fazeli A, Hasheminajafabadi S, Fazeli MR. A Novel Clot Lysis Assay for Recombinant Plasminogen Activator. Biotechnology Letters. 2015;37(3):593-600.
BibTex	@article{ author = {Jamialahmadi O and Fazeli A and Hasheminajafabadi S and Fazeli MR}, title = {A Novel Clot Lysis Assay for Recombinant Plasminogen Activator}, journal = {Biotechnology Letters}, volume = {37}, number = {3}, pages = {593-600}, year = {2015} }
RIS	TY - JOUR AU - Jamialahmadi O AU - Fazeli A AU - Hasheminajafabadi S AU - Fazeli MR TI - A Novel Clot Lysis Assay for Recombinant Plasminogen Activator JO - Biotechnology Letters VL - 37 IS - 3 SP - 593 EP - 600 PY - 2015 ER -

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