A Novel Electrochemical Immunosensor for Ultrasensitive Detection of Ca125 in Ovarian Cancer

A Novel Electrochemical Immunosensor for Ultrasensitive Detection of Ca125 in Ovarian Cancer Publisher Pubmed

Samadi Pakchin P^{1, 2} ; Fathi M² ; Ghanbari H¹ ; Saber R^{1, 3} ; Omidi Y^{2, 4}

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1. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
2. Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran
3. Research Center for Science and Technology in Medicine, Tehran University of Medical Sciences, Tehran, Iran
4. Department of Pharmaceutics, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran

Source: Biosensors and Bioelectronics Published:2020

Abstract

In the current study, we report on the design and development of a novel electrochemical immunosensor for the detection of cancer antigen 125 (CA125) oncomarker. Polyamidoamine/gold nanoparticles (PAMAM/AuNPs) were used to increase the conductivity and enhance the number of antibodies (Abs) immobilized on the electrode surface. Three-dimensional reduced graphene oxide-multiwall carbon nanotubes (3DrGO-MWCNTs) were used to modify the glassy carbon electrode to improve the electrode conductivity and specific surface area. Ab and toluidine blue attached to O-succinyl-chitosan-magnetic nanoparticles (Suc-CS@MNPs) as a tracer. The poor solubility of chitosan (CS) was improved by succinic anhydride using a novel modification method. Under optimum condition, the developed immunosensor exhibited a wide linear range (0.0005–75 U/mL) and an excellent limit of detection around 6 μU/mL. The reliability of the engineered immunosensor in detecting CA125 was verified by standard addition recovery method, which was further compared to enzyme-linked immunosorbent assay (ELISA). The proposed immunosensor exhibited excellent stability, high selectivity and sensitivity, and good reproducibility. Based on the great performance of the engineered immunosensor, it is proposed as a robust and reliable diagnostic tool for the detection of CA125 in the clinic. © 2020 Elsevier B.V.

Style	Citing Format
MLA	Samadi Pakchin P, et al.. "A Novel Electrochemical Immunosensor for Ultrasensitive Detection of Ca125 in Ovarian Cancer." Biosensors and Bioelectronics, vol. 153, no. , 2020, pp. -.
APA	Samadi Pakchin P, Fathi M, Ghanbari H, Saber R, Omidi Y (2020). A Novel Electrochemical Immunosensor for Ultrasensitive Detection of Ca125 in Ovarian Cancer. Biosensors and Bioelectronics, 153(), -.
Chicago	Samadi Pakchin P, Fathi M, Ghanbari H, Saber R, Omidi Y. "A Novel Electrochemical Immunosensor for Ultrasensitive Detection of Ca125 in Ovarian Cancer." Biosensors and Bioelectronics 153, no. (2020): -.
Harvard	Samadi Pakchin P et al. (2020) 'A Novel Electrochemical Immunosensor for Ultrasensitive Detection of Ca125 in Ovarian Cancer', Biosensors and Bioelectronics, 153(), pp. -.
Vancouver	Samadi Pakchin P, Fathi M, Ghanbari H, Saber R, Omidi Y. A Novel Electrochemical Immunosensor for Ultrasensitive Detection of Ca125 in Ovarian Cancer. Biosensors and Bioelectronics. 2020;153():-.
BibTex	@article{ author = {Samadi Pakchin P and Fathi M and Ghanbari H and Saber R and Omidi Y}, title = {A Novel Electrochemical Immunosensor for Ultrasensitive Detection of Ca125 in Ovarian Cancer}, journal = {Biosensors and Bioelectronics}, volume = {153}, number = {}, pages = {-}, year = {2020} }
RIS	TY - JOUR AU - Samadi Pakchin P AU - Fathi M AU - Ghanbari H AU - Saber R AU - Omidi Y TI - A Novel Electrochemical Immunosensor for Ultrasensitive Detection of Ca125 in Ovarian Cancer JO - Biosensors and Bioelectronics VL - 153 IS - SP - EP - PY - 2020 ER -

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