Amlodipine and Diltiazem Significantly Repress Human Rotavirus Infection in Vitro

Amlodipine and Diltiazem Significantly Repress Human Rotavirus Infection in Vitro Publisher Pubmed

Khales P¹ ; Keyvani H¹ ; Ataeipirkooh A¹ ; Saghafi MM^{2, 3} ; Bokharaeisalim F¹ ; Ghorbani S¹ ; Monavari SH¹ ; Kiani SJ¹ ; Esghaei M¹ ; Farahmand M⁴ ; Sayyahfar S⁵ ; Khanaliha K⁵ ; Habib Z¹ ; Tavakoli A⁵

Show Affiliations

1. Department of Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
2. Department of Pharmacology and Toxicology, School of Pharmacy and Pharmaceutical Sciences, Iran University of Medical Sciences, Tehran, Iran
3. Department of Pharmaceutical care, Teaching Hospital Firooz-Abadi, Iran University of Medical Sciences, Tehran, Iran
4. Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
5. Research Center of Pediatric Infectious Diseases, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran

Source: Recent Advances in Anti-Infective Drug Discovery Published:2023

Abstract

Background: Considering the role of calcium in the replication and morpho-genesis of rotaviruses, it is hypothesized that decreased cytosolic calcium levels by using calcium channel blockers can subsequently interfere with rotavirus replication. Objective: The present study investigated the effects of two calcium ion channel block-ers, amlodipine and diltiazem, against human rotavirus infection. Methods: Cytotoxic effects of the drugs on MA-104 cells were evaluated using the neu-tral red assay. The effects of amlodipine and diltiazem at non-toxic concentrations on human rotavirus were examined using cytopathic effect inhibition, TCID50, and real-time PCR assays. Results: The highest inhibitory effect was obtained at concentrations of 0.5 µg/ml of am-lodipine and 3 µg/ml of diltiazem, leading to 4.6 and 5.5 logarithmic reductions in infectious rotavirus titer and four-and a five-fold increase in the Ct values compared to the virus control, respectively (p-value < 0.001). Conversely, infectious rotavirus titers were significantly elevated compared to the virus control at concentrations above 0.9 µg/ml of amlodipine and above 25 µg/ml of diltiazem. Conclusion: Our study suggests that in addition to cardiovascular diseases, calcium channel blockers at their optimal doses may also be used to treat gastroenteritis caused by rotavirus infection. © 2023 Bentham Science Publishers.

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Style	Citing Format
MLA	Khales P, et al.. "Amlodipine and Diltiazem Significantly Repress Human Rotavirus Infection in Vitro." Recent Advances in Anti-Infective Drug Discovery, vol. 18, no. 3, 2023, pp. 205-214.
APA	Khales P, Keyvani H, Ataeipirkooh A, Saghafi MM, Bokharaeisalim F, Ghorbani S, Monavari SH, Kiani SJ, Esghaei M, Farahmand M, Sayyahfar S, Khanaliha K, Habib Z, Tavakoli A (2023). Amlodipine and Diltiazem Significantly Repress Human Rotavirus Infection in Vitro. Recent Advances in Anti-Infective Drug Discovery, 18(3), 205-214.
Chicago	Khales P, Keyvani H, Ataeipirkooh A, Saghafi MM, Bokharaeisalim F, Ghorbani S, Monavari SH, et al.. "Amlodipine and Diltiazem Significantly Repress Human Rotavirus Infection in Vitro." Recent Advances in Anti-Infective Drug Discovery 18, no. 3 (2023): 205-214.
Harvard	Khales P et al. (2023) 'Amlodipine and Diltiazem Significantly Repress Human Rotavirus Infection in Vitro', Recent Advances in Anti-Infective Drug Discovery, 18(3), pp. 205-214.
Vancouver	Khales P, Keyvani H, Ataeipirkooh A, Saghafi MM, Bokharaeisalim F, Ghorbani S, et al.. Amlodipine and Diltiazem Significantly Repress Human Rotavirus Infection in Vitro. Recent Advances in Anti-Infective Drug Discovery. 2023;18(3):205-214.
BibTex	@article{ author = {Khales P and Keyvani H and Ataeipirkooh A and Saghafi MM and Bokharaeisalim F and Ghorbani S and Monavari SH and Kiani SJ and Esghaei M and Farahmand M and Sayyahfar S and Khanaliha K and Habib Z and Tavakoli A}, title = {Amlodipine and Diltiazem Significantly Repress Human Rotavirus Infection in Vitro}, journal = {Recent Advances in Anti-Infective Drug Discovery}, volume = {18}, number = {3}, pages = {205-214}, year = {2023} }
RIS	TY - JOUR AU - Khales P AU - Keyvani H AU - Ataeipirkooh A AU - Saghafi MM AU - Bokharaeisalim F AU - Ghorbani S AU - Monavari SH AU - Kiani SJ AU - Esghaei M AU - Farahmand M AU - Sayyahfar S AU - Khanaliha K AU - Habib Z AU - Tavakoli A TI - Amlodipine and Diltiazem Significantly Repress Human Rotavirus Infection in Vitro JO - Recent Advances in Anti-Infective Drug Discovery VL - 18 IS - 3 SP - 205 EP - 214 PY - 2023 ER -

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