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Formulation, in Vitro Evaluation and Therapeutic Effect of Chitosan Coated Pluronic F127 Micelles Containing Miltefosine for the Treatment of Visceral Leishmaniasis in Domestic Dogs Naturally Infected by Leishmania Infantum Publisher Pubmed



Alizadeh Z1 ; Mohebali M1, 2 ; Aghabarari B3 ; Zarei Z1 ; Hassanpour G2 ; Eskandari SE4 ; Parsaei M5 ; Jamshidi S6 ; Foroushani AR7 ; Keshavarz H1, 2 ; Akhoundi B1 ; Heidari Z8 ; Mohebali F3 ; Yasini SP9 Show All Authors
Authors
  1. Alizadeh Z1
  2. Mohebali M1, 2
  3. Aghabarari B3
  4. Zarei Z1
  5. Hassanpour G2
  6. Eskandari SE4
  7. Parsaei M5
  8. Jamshidi S6
  9. Foroushani AR7
  10. Keshavarz H1, 2
  11. Akhoundi B1
  12. Heidari Z8
  13. Mohebali F3
  14. Yasini SP9
  15. Hassanpour A10
Show Affiliations
Authors Affiliations
  1. 1. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Nanotechnology and Advanced Materials Department, Materials and Energy Research Center (MERC), Karaj, Iran
  4. 4. Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Tabriz Health Center, Tabriz University of Medical Sciences, Tabriz, Iran
  6. 6. Department of Internal Medicine, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran
  7. 7. Department of Biostatistics and Epidemiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  8. 8. Department of Microbiology, Parasitology and Immunology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
  9. 9. Department of Veterinary Clinical Sciences, Karaj branch, Islamic Azad University, Karaj, Iran
  10. 10. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Research in Veterinary Science Published:2025


Abstract

There are a limited number of chemotherapeutic agents for the treatment canine visceral leishmaniasis (CVL). This study aimed to formulate and assess micellar formulation of miltefosine (MTF) to increase antileishmanial therapeutic effects in dogs naturally infected by Leishmania (L) infantum for the first time. MTF loaded pluronic F127 (PF/MTF) micelles were formulated and coated with chitosan (Cs/PF/MTF). Toxicity of compounds was evaluated. The in vitro and in vivo antileishmanial effect of Cs/PF/MTF was examined in L. infantum promastigotes and amastigotes as well as naturally infected dogs by L. infantum. In-vitro efficacy studies in promastigotes and amastigotes demonstrated that inhibitory concentration (IC50) of Cs/PF/MTF was significantly lower in comparison to miltefosine. In vivo data showed that Cs/PF/MTF is more effective against CVL compared with miltefosine alone. The result of this study indicated that intravenous injection of new formulation of Cs/PF/MTF was tolerated and treated on L. infantum naturally infected dogs with the dosages of 170 μg/kg daily for 28 days. © 2024
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