Comparative Study of Commercial Media to Improve Gmp Manufacturing of Recombinant Human Interferon Β-1A by Cho Cells in Perfusion Bioreactor Publisher



Sedighikamal H^{1, 2} ; Karimi Mostofi R^{1, 3} ; Sattarzadeh A¹ ; Shahbazi M¹ ; Aghazadeh H¹
Source: Cytotechnology Published:2022

Abstract

Chinese hamster ovary cells are the main cellular factories for production of a wide range of recombinant proteins in biopharmaceutical industry. Recombinant human Interferon beta-1a (rh-IFN β-1a), as a cytokine is broadly used to treat multiple sclerosis. In this work, the cell line producing rh-IFN β-1a was studied to improve cell density along with the specific expression. For this reason different cell culture experiments were done using different commercial serum-free media to find the appropriate media providing higher cell density. It was shown DMEMF12, DMEM:ProCHO5, and CHO-S-SFM II led to higher cell density and shorter doubling time. Next, using these media, fed-batch, and perfusion culture with temperature shift were implemented to investigate the best condition for industrial-scale manufacturing of rh-IFN β-1a in terms of higher cell density and product expression yield. The results demonstrated that CHO-S-SFM II media and a thermally biphasic condition provide enhanced expression of rh-IFN β-1a in perfusion bioreactor. © 2022, The Author(s), under exclusive licence to Springer Nature B.V.