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Transplantation of Mir-219 Overexpressed Human Endometrial Stem Cells Encapsulated in Fibrin Hydrogel in Spinal Cord Injury Publisher Pubmed



Jalali Monfared M1, 2 ; Nasirinezhad F3 ; Ebrahimibarough S1 ; Hasanzade G4 ; Saberi H5 ; Tavangar SM1, 6 ; Asadpour S7 ; Aryan L3 ; Barabadi Z8 ; Ai J1
Authors
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Authors Affiliations
  1. 1. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. School of Medicine, Aja University of Medical Sciences, Tehran, Iran
  3. 3. Physiology Research Center and Department of Physiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Neurosurgery, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Department of Pathology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
  7. 7. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, Iran
  8. 8. School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran

Source: Journal of Cellular Physiology Published:2019


Abstract

Oligodendrocyte (OL) loss and demyelination occur after spinal cord injury (SCI). Stimulation of remyelination through transplantation of myelinating cells may be effective in improving function. For the repair strategy to be successful, the selection of a suitable cell and maintaining cell growth when cells are injected directly to the site of injury is important. In addition to selecting the type of cell, fibrin hydrogel was used as a suitable tissue engineering scaffold for this purpose. To test the relationship between myelination and functional improvement, the human endometrial stem cells (hEnSCs) were differentiated toward oligodendrocyte progenitor cells (OPCs) using overexpression of miR-219. Adult female Wistar rats were used to induce SCI by using a compression model and were randomly assigned to the following four experimental groups: SCI, Vehicle, hEnSC, and OPC. Ten days after injury, miR-219 overexpressed hEnSC-derived OPCs encapsulated in fibrin hydrogel, as an injectable scaffold, were injected to the injury site. In this study, with a focus on promoting functional recovery after SCI, the Basso-Beattie-Bresnahan test was performed to evaluate the recovery of motor function every week for 10 weeks and the histological assay was then performed. Results showed that the rate of motor function recovery was significantly higher in OPC group compared to SCI and vehicle groups but no marked differences were found between OPC and hEnSC groups, although, the rate of myelination in the OPC group was significantly higher than the other groups. These results demonstrated that remyelination was not the cause of recovery of motor function. © 2019 Wiley Periodicals, Inc.
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