Evaluation of the Performance of Bcg With Synthetic Nano Selenium As a Booster in the Formulation of a Vaccine Candidate Against Staphylococcus Aureus Based on Isde in a Mouse Model; [ارزیابی عملکرد ب ث ژ به همراه نانو سلنیوم سنتتیک به عنوان تقویتکننده در فرمولاسیون واکسن کاندید علیه استافیلوکوکوس اورئوس بر پایهی Isde در مدل موشی] Publisher



Khosravanipour F ; Haghighat S ; Yazdi MH ; Hassanzadeh SM
Source: Journal of Isfahan Medical School Published:2025

Abstract

Background: Staphylococcus aureus causes various hospital-acquired infections and contributes to increased mortality. Vaccination is an effective method to combat these infections. Iron plays an essential role in bacterial pathogenesis. The IsdE protein, an iron (heme) transporter, is a promising vaccine development candidate. This study investigates the effect of BCG performance along with synthetic nano selenium as a booster in formulating a candidate vaccine against Staphylococcus aureus based on IsdE protein and the role of MF59 adjuvant. Methods: After expressing the IsdE protein using IPTG, a one-millimolar nickel column was used for purification. The vaccine formulation, incorporating the adjuvants MF59, BCG, and synthetic nano-selenium, was prepared and administered subcutaneously in three doses, with two-week intervals, to five groups of Balb/C mice. The levels of total IgG and IgG1 and IgG2a isotypes were measured using ELISA. The mice were challenged with a bacterial dose of 5 × 108 CFU. The bacterial load in internal organs was counted, and animal survival was monitored for 30 days. Findings: Total IgG and the IgG1 and IgG2a isotypes showed a significant increase in all groups receiving the vaccine candidate compared to the control group. The bacterial load in internal organs (liver, kidneys, and spleen) and the mortality rate in the IsdE+MF59+BCG+Nano-selenium group were significantly reduced compared to the other immunized and control groups. Conclusion: The results demonstrated that the adjuvant MF59, BCG, and synthetic nano-selenium boosters enhance humoral immunity against Staphylococcus aureus infection. © 2025, Isfahan University of Medical Sciences(IUMS). All rights reserved.