Production of Vitamin D3 Enriched Biomass of Saccharomyces Cerevisiae As a Potential Food Supplement: Evaluation and Optimization of Culture Conditions Using Plackett–Burman and Response Surface Methodological Approaches

Production of Vitamin D3 Enriched Biomass of Saccharomyces Cerevisiae As a Potential Food Supplement: Evaluation and Optimization of Culture Conditions Using Plackett–Burman and Response Surface Methodological Approaches Publisher

Amiri MM¹ ; Fazeli MR² ; Babaee T² ; Amini M³ ; Roodbari NH¹ ; Mousavi SB⁴ ; Samadi N^{2, 5}

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1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2. Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
3. Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
4. Biotechnology Group, Chemical Engineering Department, Tarbiat Modares University, Tehran, Iran
5. Pharmaceutical Quality Assurance Research Center, The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences, Tehran, Iran

Source: Iranian Journal of Pharmaceutical Research Published:2019

Abstract

Vitamin D deficiency causes osteoporosis, osteopenia, fractures, rickets, and more recently is linked with some chronic illnesses such as cancer. Because of the safety and probiotic properties of the yeast Saccharomyces cerevisiae, we hypothesized that yeast cells enriched with cholecalciferol (vitamin D3) could represent a solution for prevention or treatment of vitamin D deficiency. In this study S. cerevisiae was used as a vitamin D3 accumulator for the first time and the optimal conditions for enrichment of S. cerevisiae were determined. The Plackett-Burman screening studies were used for selection of the most important factors affecting cholecalciferol entrapment. Response surface methodology was employed for optimization of cholecalciferol accumulation in S. cerevisiae cells by using Box-Behnken design. A modified quadratic polynomial model fit the data appropriately. The optimal points of variables to maximize the response were cholecalciferol initial concentration of 358021.16 IU/mL, tryptone concentration of 1.82 g/L, sucrose concentration of 7.13 % (w/v), and shaking speed of 140.46 rpm. The maximum amount of cholecalciferol in dry cell weight of S. cerevisiae was 4428.11 IU/g. The cholecalciferol entrapment in yeast biomass increased about two-folds in optimized condition which indicates efficiency of optimization. © 2019, Iranian Journal of Pharmaceutical Research. All rights reserved.

2. Enhanced Production and Characterization of a Highly Stable Extracellular Protease From an Extreme Halophilic Isolate Salicola Marasensis, Iranian Journal of Pharmaceutical Research (2018)

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Mohammad Reza Khoshayand (1)

Mohsen Amini (1)

Style	Citing Format
MLA	Amiri MM, et al.. "Production of Vitamin D3 Enriched Biomass of Saccharomyces Cerevisiae As a Potential Food Supplement: Evaluation and Optimization of Culture Conditions Using Plackett–Burman and Response Surface Methodological Approaches." Iranian Journal of Pharmaceutical Research, vol. 18, no. 2, 2019, pp. 974-987.
APA	Amiri MM, Fazeli MR, Babaee T, Amini M, Roodbari NH, Mousavi SB, Samadi N (2019). Production of Vitamin D3 Enriched Biomass of Saccharomyces Cerevisiae As a Potential Food Supplement: Evaluation and Optimization of Culture Conditions Using Plackett–Burman and Response Surface Methodological Approaches. Iranian Journal of Pharmaceutical Research, 18(2), 974-987.
Chicago	Amiri MM, Fazeli MR, Babaee T, Amini M, Roodbari NH, Mousavi SB, Samadi N. "Production of Vitamin D3 Enriched Biomass of Saccharomyces Cerevisiae As a Potential Food Supplement: Evaluation and Optimization of Culture Conditions Using Plackett–Burman and Response Surface Methodological Approaches." Iranian Journal of Pharmaceutical Research 18, no. 2 (2019): 974-987.
Harvard	Amiri MM et al. (2019) 'Production of Vitamin D3 Enriched Biomass of Saccharomyces Cerevisiae As a Potential Food Supplement: Evaluation and Optimization of Culture Conditions Using Plackett–Burman and Response Surface Methodological Approaches', Iranian Journal of Pharmaceutical Research, 18(2), pp. 974-987.
Vancouver	Amiri MM, Fazeli MR, Babaee T, Amini M, Roodbari NH, Mousavi SB, et al.. Production of Vitamin D3 Enriched Biomass of Saccharomyces Cerevisiae As a Potential Food Supplement: Evaluation and Optimization of Culture Conditions Using Plackett–Burman and Response Surface Methodological Approaches. Iranian Journal of Pharmaceutical Research. 2019;18(2):974-987.
BibTex	@article{ author = {Amiri MM and Fazeli MR and Babaee T and Amini M and Roodbari NH and Mousavi SB and Samadi N}, title = {Production of Vitamin D3 Enriched Biomass of Saccharomyces Cerevisiae As a Potential Food Supplement: Evaluation and Optimization of Culture Conditions Using Plackett–Burman and Response Surface Methodological Approaches}, journal = {Iranian Journal of Pharmaceutical Research}, volume = {18}, number = {2}, pages = {974-987}, year = {2019} }
RIS	TY - JOUR AU - Amiri MM AU - Fazeli MR AU - Babaee T AU - Amini M AU - Roodbari NH AU - Mousavi SB AU - Samadi N TI - Production of Vitamin D3 Enriched Biomass of Saccharomyces Cerevisiae As a Potential Food Supplement: Evaluation and Optimization of Culture Conditions Using Plackett–Burman and Response Surface Methodological Approaches JO - Iranian Journal of Pharmaceutical Research VL - 18 IS - 2 SP - 974 EP - 987 PY - 2019 ER -

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