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Evaluation of Cell Adhesion Molecules (Lfa-1 and L-Selectin) in Ankylosing Spondylitis Patients After Treatment With ß-D-Mannuronic Acid (M2000) Publisher Pubmed



Fattahi MJ1, 3 ; Rehm BHA5 ; Matsuo H6 ; Cuzzocrea S7 ; Jafarnezhadansariha F1, 4 ; Ahmadi H1 ; Tofighizavareh F1, 2 ; Oraei M1 ; Aghazadeh Z1 ; Mirshafiey A1
Authors
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Authors Affiliations
  1. 1. Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Research Center for Immunodeficiencies, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
  4. 4. Department of Immunology, International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
  5. 5. Institute of Fundamental Sciences, Massey University, Palmerston North, New Zealand
  6. 6. Department of Clinical Research, Nagasaki Kawatana Medical Center, Nagasaki, Japan
  7. 7. Department of Chemical Biological, Pharmaceutical & Environmental Sciences, University of Messina, Messina, Italy

Source: Indian Journal of Medical Research Published:2023


Abstract

Background & objectives: To examine β-D-mannuronic acid (M2000) effects on L-selectin shedding and leucocyte function-associated antigen-1 (LFA-1) expression as mechanisms of action of this drug in patients with ankylosing spondylitis (AS). Methods: To investigate the molecular consequences of β-D-mannuronic acid on L-selectin shedding, flow cytometry method was used. Furthermore, the effect of it on LFA-1 gene expression was analyzed by using quantitative real time (qRT)-PCR technique. Results: The LFA-1 expression in patients with AS was higher than controls (P=0.046). The LFA-1 expression after 12 wk therapy with β-D-mannuronic acid was meaningfully decreased (P=0.01). After 12 wk treatment with β-D-mannuronic acid, the frequency of CD62L-expressing CD4+ T cells in patients with AS, was not considerably altered, compared to the patients before therapy (P=0.5). Furthermore, after 12 wk therapy with β-D-mannuronic acid, L-selectin expression levels on CD4+ T-cells in patients with AS, were not remarkably changed, compared to the expression levels of these in patients before treatment (P=0.2). Interpretation & conclusions: The results of this study for the first time showed that β-D-mannuronic acid can affect events of adhesion cascade in patients with AS. Moreover, β-D-mannuronic acid presented as an acceptable benefit to AS patients and could aid in the process of disease management. © 2023 Copyright:
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