Isolation and Structural Characterization of Coryxin, a Novel Cyclic Lipopeptide From Corynebacterium Xerosis Ns5 Having Emulsifying and Anti-Biofilm Activity

Isolation and Structural Characterization of Coryxin, a Novel Cyclic Lipopeptide From Corynebacterium Xerosis Ns5 Having Emulsifying and Anti-Biofilm Activity Publisher Pubmed

Dalili D¹ ; Amini M² ; Faramarzi MA³ ; Fazeli MR¹ ; Khoshayand MR¹ ; Samadi N¹

Show Affiliations

1. Department of Drug and Food Control, Faculty of Pharmacy and Pharmaceutical Quality Assurance Research Center, Tehran University of Medical Sciences, Tehran, Iran
2. Department of Medicinal Chemistry, Faculty of Pharmacy and Drug Design and Development Research Center, Tehran University of Medical Sciences, Tehran, Iran
3. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, Iran

Source: Colloids and Surfaces B: Biointerfaces Published:2015

Abstract

Herein we reported the structure and several properties of a new biosurfactants produced by Corynebacterium xerosis strain NS5. This strain was capable of producing a novel lipopeptide biosurfactant that we have named coryxin. The biosurfactant structure was characterized by using Fourier transform infrared spectroscopy (FTIR), Nuclear magnetic resonance spectroscopy (NMR), and Liquid chromatography-mass spectrometry (LC-MS). It contained a hydrophobic moiety of 3-hydroxydecanoic acid and a peptide part predicted as a sequence of seven amino acids including Asn-Arg-Asn-Gln-Pro-Asn-Ser. Coryxin lowered the surface tension of water to 31.4. mN/m, with a critical micelle concentration of 25 mg/l. It was a strong emulsifier with an emulsification index of 61% against n-hexane. Coryxin showed antibacterial activity against test organisms belonging to Gram-positive and Gram-negative bacteria and disrupted preformed biofilms of Staphylococcus aureus (82.5%). , Streptococcus mutans (80%), Escherichia coli (66%) and Pseudomonas aeruginosa (30%). In conclusion, microbial surfactant from C. xerosis exhibited inhibitory and disruptive activities against biofilm formation that could be of use in biofilm-related menace. © 2015 Elsevier B.V.

Style	Citing Format
MLA	Dalili D, et al.. "Isolation and Structural Characterization of Coryxin, a Novel Cyclic Lipopeptide From Corynebacterium Xerosis Ns5 Having Emulsifying and Anti-Biofilm Activity." Colloids and Surfaces B: Biointerfaces, vol. 135, no. , 2015, pp. 425-432.
APA	Dalili D, Amini M, Faramarzi MA, Fazeli MR, Khoshayand MR, Samadi N (2015). Isolation and Structural Characterization of Coryxin, a Novel Cyclic Lipopeptide From Corynebacterium Xerosis Ns5 Having Emulsifying and Anti-Biofilm Activity. Colloids and Surfaces B: Biointerfaces, 135(), 425-432.
Chicago	Dalili D, Amini M, Faramarzi MA, Fazeli MR, Khoshayand MR, Samadi N. "Isolation and Structural Characterization of Coryxin, a Novel Cyclic Lipopeptide From Corynebacterium Xerosis Ns5 Having Emulsifying and Anti-Biofilm Activity." Colloids and Surfaces B: Biointerfaces 135, no. (2015): 425-432.
Harvard	Dalili D et al. (2015) 'Isolation and Structural Characterization of Coryxin, a Novel Cyclic Lipopeptide From Corynebacterium Xerosis Ns5 Having Emulsifying and Anti-Biofilm Activity', Colloids and Surfaces B: Biointerfaces, 135(), pp. 425-432.
Vancouver	Dalili D, Amini M, Faramarzi MA, Fazeli MR, Khoshayand MR, Samadi N. Isolation and Structural Characterization of Coryxin, a Novel Cyclic Lipopeptide From Corynebacterium Xerosis Ns5 Having Emulsifying and Anti-Biofilm Activity. Colloids and Surfaces B: Biointerfaces. 2015;135():425-432.
BibTex	@article{ author = {Dalili D and Amini M and Faramarzi MA and Fazeli MR and Khoshayand MR and Samadi N}, title = {Isolation and Structural Characterization of Coryxin, a Novel Cyclic Lipopeptide From Corynebacterium Xerosis Ns5 Having Emulsifying and Anti-Biofilm Activity}, journal = {Colloids and Surfaces B: Biointerfaces}, volume = {135}, number = {}, pages = {425-432}, year = {2015} }
RIS	TY - JOUR AU - Dalili D AU - Amini M AU - Faramarzi MA AU - Fazeli MR AU - Khoshayand MR AU - Samadi N TI - Isolation and Structural Characterization of Coryxin, a Novel Cyclic Lipopeptide From Corynebacterium Xerosis Ns5 Having Emulsifying and Anti-Biofilm Activity JO - Colloids and Surfaces B: Biointerfaces VL - 135 IS - SP - 425 EP - 432 PY - 2015 ER -

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