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Chromosome Conformation Capture-Based Dna Hybridization Method for Chromosomal Translocation Screening Publisher Pubmed



Absalan M1 ; Jabbarpour Z2 ; Ghahremani MH3 ; Motevaseli E1 ; Mahmoudian F1 ; Karimi R4 ; Tavoosidana G1
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Authors Affiliations
  1. 1. Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Gene Therapy Research Center, Digestive Disease Research Institute, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Pharmacology & Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Tissue Engineering & Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Biomarkers in Medicine Published:2023


Abstract

Background: DNA probes have been widely used as diagnostic tools for translocations. This study sought to design a screening tool using ssDNA probes and chromosome conformation capture (3C) library fragment hybridization. Method: The authors focused on developing a probe for the juxtaposed region of MYC and TRD. Fragments of the MYC gene with a thiol modification (MYC-Au NP probe) were functionalized by gold nanoparticles (Au NPs). Then TRD probes were immobilized on a nitrocellulose surface. Hybridization between DNA probes and 3C library fragments of SKW3 cells was determined by color intensity. Results: Optimal hybridization of the 3C library sample of the cell line to probes showed higher color intensity than human umbilical vein endothelial cells. Conclusion: Combining 3C-based techniques and DNA-DNA hybridization can identify rearrangements in cancer cells. © 2023 Future Medicine Ltd.
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1. Application of Chromosome Conformation Capture Method for Detection Myc/Trd Chromosomal Translocation in Leukemia Cell Line, International Journal of Hematology-Oncology and Stem Cell Research (2020)
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Moloud Absalan (1)
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