Preparation of Peg-Grafted Chitosan/Streptokinase Nanoparticles to Improve Biological Half-Life and Reduce Immunogenicity of the Enzyme

Preparation of Peg-Grafted Chitosan/Streptokinase Nanoparticles to Improve Biological Half-Life and Reduce Immunogenicity of the Enzyme Publisher Pubmed

Baharifar H¹ ; Khoobi M² ; Arbabi Bidgoli S³ ; Amani A^{4, 5}

Show Affiliations

1. Department of Medical Nanotechnology, Applied Biophotonics Research Center, Science and Research Branch, Islamic Azad University, Tehran, Iran
2. Biomaterials Group, The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences, Tehran, Iran
3. Department of Toxicology and Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, Islamic Azad University, Tehran Medical Sciences (IAUTMS), Tehran, Iran
4. Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran
5. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: International Journal of Biological Macromolecules Published:2020

Abstract

Streptokinase, as a thrombolytic drug, is widely used in treatment of cardiovascular disorders and deep vein thrombosis. Streptokinase is immunogenic due to its prokaryotic source, having short biological half-life (i.e. 15 to 30 min) that is not enough for an efficient therapy. In this study, nanoparticles (NPs) of chitosan/streptokinase and polyethylene glycol (PEG)-grafted chitosan/streptokinase were prepared by polyelectrolyte complex method. Particle size of chitosan and PEG-grafted chitosan NPs were 154 ± 42 and 211 ± 47 nm, respectively. Results showed that using PEG in preparation of nanoparticles leads to ~24% decrease in encapsulation efficiency. Encapsulation of streptokinase in the NPs also resulted in a slight reduction in enzymatic activity. However, in vivo findings indicated that response of the immune system was delayed for 20 days and blood circulation time of the enzyme increased up to 120 min by using PEG. Biological half-life of the drug also increased up to twice in PEG-grafted chitosan. In conclusion, PEG-grafted chitosan NPs could be an alternative for delivery of streptokinase to reduce its clinical limitations. © 2019

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Style	Citing Format
MLA	Baharifar H, et al.. "Preparation of Peg-Grafted Chitosan/Streptokinase Nanoparticles to Improve Biological Half-Life and Reduce Immunogenicity of the Enzyme." International Journal of Biological Macromolecules, vol. 143, no. , 2020, pp. 181-189.
APA	Baharifar H, Khoobi M, Arbabi Bidgoli S, Amani A (2020). Preparation of Peg-Grafted Chitosan/Streptokinase Nanoparticles to Improve Biological Half-Life and Reduce Immunogenicity of the Enzyme. International Journal of Biological Macromolecules, 143(), 181-189.
Chicago	Baharifar H, Khoobi M, Arbabi Bidgoli S, Amani A. "Preparation of Peg-Grafted Chitosan/Streptokinase Nanoparticles to Improve Biological Half-Life and Reduce Immunogenicity of the Enzyme." International Journal of Biological Macromolecules 143, no. (2020): 181-189.
Harvard	Baharifar H et al. (2020) 'Preparation of Peg-Grafted Chitosan/Streptokinase Nanoparticles to Improve Biological Half-Life and Reduce Immunogenicity of the Enzyme', International Journal of Biological Macromolecules, 143(), pp. 181-189.
Vancouver	Baharifar H, Khoobi M, Arbabi Bidgoli S, Amani A. Preparation of Peg-Grafted Chitosan/Streptokinase Nanoparticles to Improve Biological Half-Life and Reduce Immunogenicity of the Enzyme. International Journal of Biological Macromolecules. 2020;143():181-189.
BibTex	@article{ author = {Baharifar H and Khoobi M and Arbabi Bidgoli S and Amani A}, title = {Preparation of Peg-Grafted Chitosan/Streptokinase Nanoparticles to Improve Biological Half-Life and Reduce Immunogenicity of the Enzyme}, journal = {International Journal of Biological Macromolecules}, volume = {143}, number = {}, pages = {181-189}, year = {2020} }
RIS	TY - JOUR AU - Baharifar H AU - Khoobi M AU - Arbabi Bidgoli S AU - Amani A TI - Preparation of Peg-Grafted Chitosan/Streptokinase Nanoparticles to Improve Biological Half-Life and Reduce Immunogenicity of the Enzyme JO - International Journal of Biological Macromolecules VL - 143 IS - SP - 181 EP - 189 PY - 2020 ER -

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