Developing a Dna Aptamer-Based Approach for Biosensing Cystatin-C in Serum: An Alternative to Antibody-Based Methods

Developing a Dna Aptamer-Based Approach for Biosensing Cystatin-C in Serum: An Alternative to Antibody-Based Methods Publisher Pubmed

Kooshki H¹ ; Abbaszadeh R¹ ; Heidari R¹ ; Akbariqomi M¹ ; Mazloumi M² ; Shafei S¹ ; Absalan M¹ ; Tavoosidana G¹

Show Affiliations

1. Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
2. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Analytical Biochemistry Published:2019

Abstract

Oligonucleotide aptamers are short, synthetic and single-stranded DNA or RNA molecules capable of binding to a wide range of molecules, from small molecules to large cells. Nowadays, aptamers are valuable tools in research, clinical diagnosis and treatment. Their small size and high specificity in addition to their lack of immunogenicity make them great alternatives to other diagnosing candidates such as antibodies. In this study, we have introduced a new method based on competitive Enzyme-Linked Aptamer Sorbent Assay (ELASA) using single-stranded DNA (ssDNA) aptamers to measure cystatin-c levels in serum samples. To this aim, through a Systematic Evolution of Ligands by Exponential Enrichment (SELEX) process a number of aptamers were selected from which an aptamer with a Kd (dissociation constant) value of 65.5 ± 0.007 nM was chosen for further analyses. The limit of detection (LoD) was found to be 216.077 pg/ml. The results of the analytical application of this method in serum samples were comparable to those of commonly used commercial kits. © 2019 Elsevier Inc.

2. Staggered Target Selex, a Novel Approach to Isolate Non-Cross-Reactive Aptamer for Detection of Sea by Apta-Qpcr, Journal of Biotechnology (2018)

Experts (# of related papers)

Mohsen Amin (1)

Style	Citing Format
MLA	Kooshki H, et al.. "Developing a Dna Aptamer-Based Approach for Biosensing Cystatin-C in Serum: An Alternative to Antibody-Based Methods." Analytical Biochemistry, vol. 584, no. , 2019, pp. -.
APA	Kooshki H, Abbaszadeh R, Heidari R, Akbariqomi M, Mazloumi M, Shafei S, Absalan M, Tavoosidana G (2019). Developing a Dna Aptamer-Based Approach for Biosensing Cystatin-C in Serum: An Alternative to Antibody-Based Methods. Analytical Biochemistry, 584(), -.
Chicago	Kooshki H, Abbaszadeh R, Heidari R, Akbariqomi M, Mazloumi M, Shafei S, Absalan M, Tavoosidana G. "Developing a Dna Aptamer-Based Approach for Biosensing Cystatin-C in Serum: An Alternative to Antibody-Based Methods." Analytical Biochemistry 584, no. (2019): -.
Harvard	Kooshki H et al. (2019) 'Developing a Dna Aptamer-Based Approach for Biosensing Cystatin-C in Serum: An Alternative to Antibody-Based Methods', Analytical Biochemistry, 584(), pp. -.
Vancouver	Kooshki H, Abbaszadeh R, Heidari R, Akbariqomi M, Mazloumi M, Shafei S, et al.. Developing a Dna Aptamer-Based Approach for Biosensing Cystatin-C in Serum: An Alternative to Antibody-Based Methods. Analytical Biochemistry. 2019;584():-.
BibTex	@article{ author = {Kooshki H and Abbaszadeh R and Heidari R and Akbariqomi M and Mazloumi M and Shafei S and Absalan M and Tavoosidana G}, title = {Developing a Dna Aptamer-Based Approach for Biosensing Cystatin-C in Serum: An Alternative to Antibody-Based Methods}, journal = {Analytical Biochemistry}, volume = {584}, number = {}, pages = {-}, year = {2019} }
RIS	TY - JOUR AU - Kooshki H AU - Abbaszadeh R AU - Heidari R AU - Akbariqomi M AU - Mazloumi M AU - Shafei S AU - Absalan M AU - Tavoosidana G TI - Developing a Dna Aptamer-Based Approach for Biosensing Cystatin-C in Serum: An Alternative to Antibody-Based Methods JO - Analytical Biochemistry VL - 584 IS - SP - EP - PY - 2019 ER -

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