Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
The Cooperative Effects of Micro-Grooved Topography and Tgf-Β1 on the Vascular Smooth Muscle Cell Contractile Protein Expression of the Mesenchymal Stem Cells Publisher Pubmed



Abolhasani S1 ; Rajabibazl M1 ; Khani MM2 ; Parandakh A3 ; Hoseinpoor R4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Clinical Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  2. 2. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  3. 3. Faculty of Biomedical Engineering, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran
  4. 4. Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Differentiation Published:2020


Abstract

Cell morphological changes induced by micro-grooved topography have been shown to be an important regulator of smooth muscle (SM) differentiation of mesenchymal stem cells (MSCs). In addition to the micro-grooved topography, transforming growth factor-β1 (TGF-β1) can also modulate MSCs differentiation towards smooth muscle cells (SMCs) through alterations in cell morphological characteristics. Thus, it can be hypothesized that substrate topography and TGF-β1 may interact to facilitate differentiation of MSCs into SMCs. In this study, we investigated the time-course cooperative effects of substrate topography and TGF-β1 in the regulation of SM differentiation of human MSCs. Western blotting, followed by image analysis, was performed to assess the protein expression of α-actin, h1-calponin and gelsolin. Three-way analysis of variance was employed to investigate the main effect of each independent variable, i.e. TGF-β1 conditioning, substrate topography and culture time, along with the interactions of these variables. Each of TGF-β1, substrate topography and culture time significantly affected the protein expression of α-actin, h1-calponin and gelsolin. Overall, TGF-β1 conditioning of the cells and culturing the cells on the micro-grooved substrate resulted in greater protein expression of α-actin and h1-calponin, and lesser protein expression of gelsolin. In addition to the isolated effects of the variables, treatment type interacted with substrate topography and culture time to regulate the expression of the above-mentioned proteins. This study indicated the feasibility of promoting SM differentiation of human MSCs by simultaneous recruitment of micro-grooved topography and TGF-β1. The findings could be of assistance when effective utilization of chemo-physical cues is needed to achieve functional SMC-like MSCs in vitro. © 2020 International Society of Differentiation
Related Docs
1. Substrate Topography Interacts With Substrate Stiffness and Culture Time to Regulate Mechanical Properties and Smooth Muscle Differentiation of Mesenchymal Stem Cells, Colloids and Surfaces B: Biointerfaces (2019)
2. Morphology and Contractile Gene Expression of Adipose-Derived Mesenchymal Stem Cells in Response to Short-Term Cyclic Uniaxial Strain and Tgf-Β1, Biomedizinische Technik (2018)
3. Effects of Cyclic Uniaxial Strain on Morphology of Mesenchymal Stem Cells During Differentiation to Smooth Muscle Cells, Koomesh (2016)
4. The Effects of Short-Term Uniaxial Strain on the Mechanical Properties of Mesenchymal Stem Cells Upon Tgf-Β1 Stimulation, In Vitro Cellular and Developmental Biology - Animal (2018)
5. Mechanical Characterization of Human Mesenchymal Stem Cells Subjected to Cyclic Uniaxial Strain and Tgf-Β1, Journal of the Mechanical Behavior of Biomedical Materials (2015)