Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
The Comparative Effect of Plasma Exosomes of Young and Old People on the Expression of Bcl-2 and Bax Genes in Hematopoietic Stem Cells Publisher



Moradi R1 ; Afrisham R2 ; Kashanikhatib Z1 ; Mousavi SH1 ; Soleimani M3 ; Alizadeh S1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Hematology and Transfusion Sciences, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Applied Cell Sciences and Hematology Department, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Source: Indian Journal of Hematology and Blood Transfusion Published:2024


Abstract

Apoptosis may disrupt differentiation of hematopoietic stem cells (HSCs), which can affect aging. Thus, the main goal of this study was to compare the effect of plasma exosomes from young and old people on the expression of Bcl-2-associated X (BAX) and B-cell lymphoma 2 (BCL-2) genes in the HSCs. Plasma samples were acquired from four elderly adults and four younger adults, referring to Blood Transfusion Organization of Tehran-Iran during August 2022– September 2022.Then, the exosomes of the samples were extracted and analyzed using DLS, TEM, and CD63 surface marker. HSCs were isolated from umbilical cord blood cells. The MTT test was used to assess the viability of exosomes-treated HSCs at doses of 5 and 10 μg/ml. The expression of BAX and BCL-2 genes in the cells was examined using real-time PCR. A one-way analysis of variance (ANOVA) was performed to examine the distinctions among five groups. The viability of HSCs was not affected by the exosomes from young and old people than the control group (P = 0.453). Exosomes from young people (doses 5 and 10 µg/ml) did not have any significant impacts on BAX (P = 0.746, and P = 0.345, respectively) and BCL-2 (P = 0.773, and P = 0.461, respectively) expression in the HSCs compared to the control group. The BAX gene was significantly upregulated and the BCL-2 gene was significantly downregulated after utilizing the exosomes derived from the plasma of elderly individuals (dose 10 µg/ml) compared to the control (P = 0.001, P = 0.002, respectively). The current research shows that aged people's exosomes can increase BAX/ BCL-2 ratio in umbilical cord blood-derived HSCs compared to control and young groups. © Indian Society of Hematology and Blood Transfusion 2024.
Related Docs
1. Exosome-Mediated Regulation of Inflammatory Pathway During Respiratory Viral Disease, Virology Journal (2024)
2. Contribution of Extracellular Vesicles and Molecular Chaperones in Age-Related Neurodegenerative Disorders of the Cns, International Journal of Molecular Sciences (2023)
3. Review of Mesenchymal Stem Cell-Derived Exosomes and Their Potential Therapeutic Roles in Treating Rheumatoid Arthritis, Molecular Biology Reports (2025)
4. Exosome Application in Treatment and Diagnosis of B-Cell Disorders: Leukemias, Multiple Sclerosis, and Arthritis Rheumatoid, Cellular and Molecular Biology Letters (2022)
5. Comparison of the Characteristics of Circulating Small Extracellular Vesicles Isolated by Ultracentrifugation and a Commercial Kit, Recent Patents on Biotechnology (2025)
6. Current Understanding of the Exosomes and Their Associated Biomolecules in the Glioblastoma Biology, Clinical Treatment, and Diagnosis, Journal of Neuroimmune Pharmacology (2025)
Experts (# of related papers)
View all Related Experts
Shaban Alizadeh (1)
Reza Afrisham (1)