Assessment of Cytotoxic Damage Induced by Irradiation Combined With Hyperthermia and Gemcitabine on Cultured Glioblastoma Spheroid Cells

Assessment of Cytotoxic Damage Induced by Irradiation Combined With Hyperthermia and Gemcitabine on Cultured Glioblastoma Spheroid Cells Publisher

Asayesh T¹ ; Changizi V¹ ; Eyvazzadeh N²

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1. Technology of Radiology and Radiotherapy Department, Allied Medical Sciences School, Tehran University of Medical Sciences, Tehran, Iran
2. Radiation Research Center, Allied Medical Sciences School, AJA University of Medical Sciences, Tehran, Iran

Source: Radiation Physics and Chemistry Published:2016

Abstract

Background: Glioblastoma is a highly lethal tumor. There are several methods for the treatment of glioblastoma including surgery/chemotherapy/radiotherapy/radiosurgery or corticosteroids. However none of them could have improved treatment considerably. Therefore this study assessed the cytotoxicity of the combined treatment with Gemcitabine, radiation and hyperthermia on cultured glioblastoma spheroid cells. Material and methods: In this study human glioblastoma cell line U87-MG, was cultured as spheroid using the liquid overlay technique. After reaching spheroid dimensions to 100. μm, cells treated by 100. nM Gemcitabine, 2. Gy gamma rays and then hyperthermia of 43. °C for an hour. Colony assay was used to determine cell induced damage and synergistic effect of Gemcitabine, irradiation and hyperthermia. Results: The combined therapy indicated greater percentage of cellular damage, although more damages were seen when Gemcitabine, radiation and hyperthermia induced into U87-MG cell line simultaneously. Combination of those three factors in comparison to the radiation combined with hyperthermia and Gemcitabine separately showed 1.85 and 1.5 times more damages respectively. Conclusion: This study revealed the synergistic effect for the combined therapy of Gemcitabine, radiation and hyperthermia in glioblastoma treatment. © 2015 Elsevier Ltd.

Style	Citing Format
MLA	Asayesh T, Changizi V, Eyvazzadeh N. "Assessment of Cytotoxic Damage Induced by Irradiation Combined With Hyperthermia and Gemcitabine on Cultured Glioblastoma Spheroid Cells." Radiation Physics and Chemistry, vol. 120, no. , 2016, pp. 44-48.
APA	Asayesh T, Changizi V, Eyvazzadeh N (2016). Assessment of Cytotoxic Damage Induced by Irradiation Combined With Hyperthermia and Gemcitabine on Cultured Glioblastoma Spheroid Cells. Radiation Physics and Chemistry, 120(), 44-48.
Chicago	Asayesh T, Changizi V, Eyvazzadeh N. "Assessment of Cytotoxic Damage Induced by Irradiation Combined With Hyperthermia and Gemcitabine on Cultured Glioblastoma Spheroid Cells." Radiation Physics and Chemistry 120, no. (2016): 44-48.
Harvard	Asayesh T, Changizi V, Eyvazzadeh N (2016) 'Assessment of Cytotoxic Damage Induced by Irradiation Combined With Hyperthermia and Gemcitabine on Cultured Glioblastoma Spheroid Cells', Radiation Physics and Chemistry, 120(), pp. 44-48.
Vancouver	Asayesh T, Changizi V, Eyvazzadeh N. Assessment of Cytotoxic Damage Induced by Irradiation Combined With Hyperthermia and Gemcitabine on Cultured Glioblastoma Spheroid Cells. Radiation Physics and Chemistry. 2016;120():44-48.
BibTex	@article{ author = {Asayesh T and Changizi V and Eyvazzadeh N}, title = {Assessment of Cytotoxic Damage Induced by Irradiation Combined With Hyperthermia and Gemcitabine on Cultured Glioblastoma Spheroid Cells}, journal = {Radiation Physics and Chemistry}, volume = {120}, number = {}, pages = {44-48}, year = {2016} }
RIS	TY - JOUR AU - Asayesh T AU - Changizi V AU - Eyvazzadeh N TI - Assessment of Cytotoxic Damage Induced by Irradiation Combined With Hyperthermia and Gemcitabine on Cultured Glioblastoma Spheroid Cells JO - Radiation Physics and Chemistry VL - 120 IS - SP - 44 EP - 48 PY - 2016 ER -

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