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Effects of Dental Pulp Stem Cell Preconditioning on Osteogenesis Using Conditioned Media of Probiotics Bacteria Publisher



Amini F1 ; Rezvani MB2 ; Bakhtiari R3 ; Tabatabaei Ghomsheh E2
Authors
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Authors Affiliations
  1. 1. School of Dentistry, Shahed University of Medical Sciences, Tehran, Iran
  2. 2. Department of Restorative Dentistry, School of Dentistry, Shahed University, Tehran, Iran
  3. 3. Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Source: Avicenna Journal of Medical Biotechnology Published:2023


Abstract

Background: Stem cells are used to treat numerous diseases; however, their lifespan is rather short. Factors such as probiotics affect and improve various cell lineage effica-cies. The aim of this study was to investigate the effects of probiotics-conditioned media on dental pulp stem cell potentials in osteogenesis. Methods: The experiment was initiated by culturing Lactobacillus casei and Lactoba-cillus acidophilus probiotics as well as DPS-7 cells. Bacterial supernatants were sepa-rated and concentrated as the conditioned media. The DPS-7 cells were treated with various concentrations of the conditioned media. Furthermore, MTT assay and alkaline phosphatase activity were used. The mRNA expression of three genes (bFGF, EGF-β and BMP-2) involved in osteogenesis was analyzed using a real-time polymerase chain reaction. Results: The response of dental pulp stem cells to probiotics preconditioning promoted cell proliferation, increased alkaline phosphatase activity and upregulated bFGF and BMP-2 gene expression. Increased expression was significant for BMP-2 and moderate for bFGF; however, it was non-significant for EGF-β. The use of the two probiotics was the most effective. Conclusion: In general, synergism of the combined probiotics preconditioning induces differentiation of DPS-7 cells into osteoblasts most effectively. © 2023, Avicenna Journal of Medical Biotechnology. All rights reserved.