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Platelet Transfusion Outcome and Flow Cytometric Monocyte Phagocytic Assay (Fmpa) Pubmed



Sayyadi M1 ; Shaiegan M2 ; Zarif MN2 ; Vaezi M3 ; Mohammadi S4
Authors
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Authors Affiliations
  1. 1. High Institute for Research and Education in Transfusion Medicine, Immunohematology Department, Tehran, Iran
  2. 2. Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Immunohematology Department, Tehran, Iran
  3. 3. Hematology/Oncology Department, Hematology, Oncology and Stem Cell Transplantation Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Hematology, Oncology and Stem Cell Transplantation Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran

Source: Archives of Iranian Medicine Published:2016


Abstract

Background: This study was designed to evaluate platelet transfusion outcome via flow cytometric monocyte phagocytic assay (FMPA). Method: Fifteen patients with a history of multiple platelet transfusions and fifteen controls were enrolled in this study. CMFDA-labeled platelets were incubated with patients’ sera and were Anally incubated with monocytes in a tube and analyzed by flow cytometry. Monocytes that phagocytosed platelets were detected as a CMFDA-positive platelet population via monocyte gate. The FMPA results were compared with CCI results for the patients. Result: The FMPA result correlated with 1 -hour (r = -0.885, P = 0.001) and 24-hour (r = -0.884, P = 0.001) CCI. There is a significant difference in means of FMPA results between the patients with immune platelet refractoriness (68.46 ± 10.4%), non-refractory group (37.73 ± 15.21%) and the control group (18.27 ± 2.86%). Conclusion: Our data showed that FMPA has good results in evaluation of platelet transfusion outcome and may be useful as an indicator of platelet transfusion response. © 2016, Academy of Medical Sciences of the I.R. Iran.
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