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Analytical Characterization of Label-Free Immunosensor Subsystems Based on Multi-Walled Carbon Nanotube Array-Modified Gold Interface Publisher Pubmed



Zahmatkeshan M1 ; Ilkhani H2 ; Paknejad M3 ; Adel M1 ; Sarkar S1, 4 ; Saber R1, 4
Authors
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Authors Affiliations
  1. 1. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences (TUMS), Tehran, Iran
  2. 2. Department of Chemistry, School of Science, University of Guilan, Rasht, Iran
  3. 3. Department of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Nanotechnology Group, Research Center for Science and Technology in Medicine (RCSTIM), Tehran University of Medical Sciences (TUMS), P.O. Box 1978647653, Tehran, Iran

Source: Combinatorial Chemistry and High Throughput Screening Published:2015


Abstract

In the present work, we report characterization studies of antigen(Ag)-antibody (Ab) interaction based on the multi-walled carbon nanotube array-modified gold electrode for label-free electrochemical immunosensor.-COOH functionalization of MWCNT was suggested by FTIR spectroscopy. Images from atomic force microscopy (AFM) and RAMAN spectroscopy confirmed the interaction of Ab on the gold interface based on the MWCNT array. A cyclic voltammetric study exhibited a linear response in phosphate buffered saline (PBS) solution including 1mM K3Fe (CN) 6 at pH 7.4 with PSA concentrations over a range of 2 to 40 ngmL-1, and a calculated detection limit of 0.56 ngmL-1. © 2015 Bentham Science Publishers.