Rapid Identification of Mycobacterium Species Isolated From Clinical Specimens of City Jahrom by Real Time Pcr Publisher



Yazdi HR¹ ; Azadbakht M^{1, 2} ; Foruozandeh H^{2, 3} ; Ardakani MT² ; Zarei E⁴ Show Affiliations
Source: Gene Reports Published:2020

Abstract

Diagnosis and identification of mycobacterial infections is often a challenge that engages different medical fields. Due to the time-consuming diagnostic procedures for most mycobacteria and the lack of rapid differentiation between Mycobacterium tuberculosis and NTMs species, incorrect diagnosis of non-tuberculosis leads to the use of TB treatment protocol and prolonged hospitalization. So, the aim of this study was to rapid identification of Mycobacterium tuberculosis strains from non-Mycobacterial tuberculosis by the multiplex Real time PCR method to prevent misdiagnosis in the treatment procedures. In this study, 82 sputum specimens were collected from patients suspected to tuberculosis referred to Peymanieh Hospital and health centers of Jahrom, as well as those treated with TB drugs referred to Jahrom Tuberculosis Center in 2015. Sediment was collected from the clinical samples by Petroff's method, DNA extraction was done with commercial DNA kit. 10 pairs primer; 23srRNA, grvB, ITS 152 bp, ITS172bp 164 bp, ITS, dnaJ, katG, rpoB, ITS 185 bp, ITS 185 bp were used. Multiplex real time PCR was performed in one run and in three different reactions and their melting curve was evaluated for both species and drug resistance. Among 82 suspected tuberculosis patients referred to Jahrom Tuberculosis Center, 26 (31.7% of total samples) were Mycobacterium positive, of which 13 (15.85%) were Mycobacterium tuberculosis, 7 (53.58%) Mycobacterium kansasii, 1 specimen (1.21%) were Mycobacterium gordonae and 5 specimens (6.09%) were uncommon Mycobacterium tuberculosis. No MDR was found in Mycobacterium tuberculosis specimens and no non-tuberculosis resistant specimens were found in Mycobacterium tuberculosis specimens. According to the results of this study, the prevalence of Mycobacterium tuberculosis and non-tuberculosis in Jahrom city was different from other parts of the country. Also, this procedure is convenient, easy and quick to detect common mycobacterial strains to prevent ectopic treatments and is also a good way to follow up treatment in treated cases. © 2020 Elsevier Inc.