The Decrease in Nkg2d+ Natural Killer Cells in Peripheral Blood of Patients With Metastatic Colorectal Cancer

The Decrease in Nkg2d+ Natural Killer Cells in Peripheral Blood of Patients With Metastatic Colorectal Cancer Publisher Pubmed

Gharagozloo M¹ ; Kalantari H² ; Rezaei A¹ ; Maracy MR³ ; Salehi M⁴ ; Bahador A⁵ ; Hassannejad N⁶ ; Narimani M⁷ ; Sanei MH⁸ ; Bayat B⁹ ; Ghazanfari H¹

Show Affiliations

1. Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2. Department of Gastroenterology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3. Department of Biostatistics and Epidemiology, School of Public Health, Isfahan University of Medical Sciences, Isfahan, Iran
4. Department of Genetics and Molecular Biology, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran
5. Department of Microbiology, Faculty of medicine, Tehran University of Medical Sciences, Tehran, Iran
6. Department of cellular and molecular biology, Faculty of Sciences, Science and Research Branch of Islamic Azad University, Tehran, Iran
7. Central lab, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran
8. Department of Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
9. Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran

Source: Bratislava Medical Journal Published:2015

Abstract

Background: Natural killer (NK) cells play important roles in the immune defense against tumors such as colorectal cancer. In humans, NKG2D is an activating immune receptor constitutively expressed in most cytotoxic lymphocytes including NK and CD8+ T cells. In this study, the expression of NKG2D molecule was investigated in peripheral blood NK cells from colorectal cancer patients and compared with healthy subjects. Methods: We studied 21 non-metastatic (low-grade), 17 non-metastatic (high-grade), 16 metastatic colorectal cancer patients, and 24 healthy controls. Peripheral blood samples were obtained to isolate peripheral blood mononuclear cells (PBMCs) and the percentage of peripheral blood NKG2D+CD3-CD56+ NK cells was analyzed by flow cytometry. The expression of NKG2D at mRNA level was also measured by real-time PCR in both, patients and control subjects. Results: The results showed a significant reduction in the percentage of NKG2D+NK cells as well as NKG2D mRNA expression in peripheral blood of metastatic colon cancer patients. Conclusion: This result suggests that decreased expression of activating NKG2D receptor in metastatic colorectal cancer might compromise NK cell function and allow tumor to evade immunity (Tab. 3, Fig. 4, Ref. 33). Text in PDF www.elis.sk.

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Style	Citing Format
MLA	Gharagozloo M, et al.. "The Decrease in Nkg2d+ Natural Killer Cells in Peripheral Blood of Patients With Metastatic Colorectal Cancer." Bratislava Medical Journal, vol. 116, no. 5, 2015, pp. 296-301.
APA	Gharagozloo M, Kalantari H, Rezaei A, Maracy MR, Salehi M, Bahador A, Hassannejad N, Narimani M, Sanei MH, Bayat B, Ghazanfari H (2015). The Decrease in Nkg2d+ Natural Killer Cells in Peripheral Blood of Patients With Metastatic Colorectal Cancer. Bratislava Medical Journal, 116(5), 296-301.
Chicago	Gharagozloo M, Kalantari H, Rezaei A, Maracy MR, Salehi M, Bahador A, Hassannejad N, et al.. "The Decrease in Nkg2d+ Natural Killer Cells in Peripheral Blood of Patients With Metastatic Colorectal Cancer." Bratislava Medical Journal 116, no. 5 (2015): 296-301.
Harvard	Gharagozloo M et al. (2015) 'The Decrease in Nkg2d+ Natural Killer Cells in Peripheral Blood of Patients With Metastatic Colorectal Cancer', Bratislava Medical Journal, 116(5), pp. 296-301.
Vancouver	Gharagozloo M, Kalantari H, Rezaei A, Maracy MR, Salehi M, Bahador A, et al.. The Decrease in Nkg2d+ Natural Killer Cells in Peripheral Blood of Patients With Metastatic Colorectal Cancer. Bratislava Medical Journal. 2015;116(5):296-301.
BibTex	@article{ author = {Gharagozloo M and Kalantari H and Rezaei A and Maracy MR and Salehi M and Bahador A and Hassannejad N and Narimani M and Sanei MH and Bayat B and Ghazanfari H}, title = {The Decrease in Nkg2d+ Natural Killer Cells in Peripheral Blood of Patients With Metastatic Colorectal Cancer}, journal = {Bratislava Medical Journal}, volume = {116}, number = {5}, pages = {296-301}, year = {2015} }
RIS	TY - JOUR AU - Gharagozloo M AU - Kalantari H AU - Rezaei A AU - Maracy MR AU - Salehi M AU - Bahador A AU - Hassannejad N AU - Narimani M AU - Sanei MH AU - Bayat B AU - Ghazanfari H TI - The Decrease in Nkg2d+ Natural Killer Cells in Peripheral Blood of Patients With Metastatic Colorectal Cancer JO - Bratislava Medical Journal VL - 116 IS - 5 SP - 296 EP - 301 PY - 2015 ER -

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