Subtilisin Gene Activity in Dermatophytes: A Study on the Presence of the Subtilisin Gene in Trichophyton Verrucosum and Microsporum Gypseum in Clinical and Nonclinical Samples in Tehran, Iran Publisher Pubmed



Naeimipour F¹ ; Hashemi SJ² ; Rezaie S² ; Bayat M¹ Show Affiliations
Source: Archives of Razi Institute Published:2021

Abstract

The keratinolytic activities of dermatophyte species are accompanied by the secretion of enzymes, such as serine proteases, which are coded by the Subtilisin (SUB) genes. This study aimed to determine the presence of the SUB genes in the clinical and nonclinical samples of Trichophyton verrucosum and Microsporum gypseum. Isolation was carried out by direct and laboratory examination. Following that, for the determination of the presence of the SUB gene, polymerase chain reaction with specific primers was conducted. The frequencies of the SUB gene were observed in almost 66% of the isolates. Statistical analysis showed a significant relationship between the presence of the SUB gene and the samples collected from human, animals, and soil (P<0.005). The current investigation has been the first study of the presence/absence of the SUB gene in the clinical and nonclinical isolates of T. verrucosum and M. gypseum in Iran which may be a new step to perform further studies. © 2021 Razi Vaccine and Serum Research Institute. All rights reserved.