Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
An in Vitro Method to Survey Dna Methylation in Peripheral Blood Mononuclear Cells (Pbmcs) Treated by Airborne Particulate Matter (Pm10) Publisher



Faraji M1 ; Pourpak Z2 ; Naddafi K1, 3 ; Nabizadeh Nodehi R1, 3 ; Nicknam MH4 ; Shamsipour M5 ; Alizadeh Z2 ; Rezaei S6 ; Soleimanifar N4 ; Mesdaghinia A1, 3
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Center for Air Pollution Research (CAPR), Institute for Environmental Research (IER), Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Molecular Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Research Methodology and Data Analysis, Institute for Environmental Research, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Social Determinants of Health Research Center, Yasuj University of Medical Sciences, Yasuj, Iran

Source: MethodsX Published:2018


Abstract

The International Agency for Research on Cancer (IARC) has defined outdoor air pollution and PM as the human carcinogen (Group 1), which mainly cause an increased risk of lung cancer. Scientists have considered epigenetic modifications as a possible mechanism to deal with adverse effects of air pollution. This study aimed to compare the effect of airborne PM10 (PM with aerodynamic diameter ≤10 μm) on in vitro global methylation in human peripheral blood mononuclear cells (PBMCs). PM10 was sampled in metropolitan Tehran, the capital of Iran. PBMCs were extracted from whole blood of healthy males and treated with PM10 suspension at concentrations of 50–300 μg/mL for 4 h. Untreated cells were used as the negative control. Genomic DNA was extracted from each sample using the DNA blood mini kit according to the manufacturer's instruction. Moreover, 5-methylsytosine (%5-mC) and 5-hydroxymethylcytosine (%5-hmC) were measured by the enzyme-linked immunosorbent assay (ELISA) method. %5-mC and %5-hmC in each sample was compared with negative control and reported as difference %5-mC and %5-hmC. © 2018 The Author(s)
Related Docs
1. Chemical Composition of Pm10 and Its Effect on in Vitro Hemolysis of Human Red Blood Cells (Rbcs): A Comparison Study During Dust Storm and Inversion, Journal of Environmental Health Science and Engineering (2019)
2. Effects of Airborne Particulate Matter (Pm10) From Dust Storm and Thermal Inversion on Global Dna Methylation in Human Peripheral Blood Mononuclear Cells (Pbmcs) in Vitro, Atmospheric Environment (2018)
3. Proinflammatory Effects of Dust Storm and Thermal Inversion Particulate Matter (Pm10) on Human Peripheral Blood Mononuclear Cells (Pbmcs) in Vitro: A Comparative Approach and Analysis, Journal of Environmental Health Science and Engineering (2019)
4. An in Vitro Method to Evaluate Hemolysis of Human Red Blood Cells (Rbcs) Treated by Airborne Particulate Matter (Pm 10 ), MethodsX (2019)
5. Investigation and Comparison of in Vitro Genotoxic Potency of Pm10 Collected in Rural and Urban Sites at Tehran in Different Metrological Conditions and Different Seasons, Biological Trace Element Research (2019)
6. Carcinogenic Risks and Chemical Composition of Particulate Matter Recovered by Two Methods: Wet and Dry Extraction, Environmental Monitoring and Assessment (2020)
Experts (# of related papers)
View all Related Experts
Kazem Naddafi (6)
Zahra Pourpak (4)