Expression of D2-40 and Cd105 Markers in Oral and Dermal Melanoma



Naeini FB¹ ; Monshizadeh R¹ ; Rahrotaban S¹ Show Affiliations
Source: Annals of Tropical Medicine and Public Health Published:2018

Abstract

Background: Diagnosing lymph angiogenesis and tumor angiogenesis and understanding its correlation with early diagnosis of tumor metastasis to lymph node is of great importance considering the increasing trend of the occurrence of malignant melanoma. The present research seeks to study the expression of D2-40 markers (as marker of Lymphangiogenesis) and CD105 (as marker of Angiogenesis) in dermal and oral melanoma. Materials and Method: This is a cross sectional-descriptive research conducted on 60 pa (30 with oral melanoma and 30 with dermal melanoma) resorting to pathology unit of Imam Khomeini Hospital's Cancer Institute from 2003 to 2014. Using Immunohistochemistry method based upon CD105 and D2-40 antibodies, the processes of angiogenesis and lymphangiogenesis inside and around the tumor in dermal and oral melanoma were studied and statistically compared against one another. The level of significance in this research was set to P-value < 0.05. Results: The present research was conducted on 60 paraffin blocks of related patients (47 males and 13 females) with an average age of 72.68 ± 8.246 years old. As the assessments demonstrated, the lymphatic vessels outnumbered blood vessels in both types of melanoma. A comparison between angiogenesis and lymphangiogenesis in oral and dermal melanoma in terms of the place of angiogenesis demonstrated that the procedure of angiogenesis around and inside the tumor in oral melanoma is significantly more than what was observed in dermal melanoma (P-value < 0.05). A direct and statistically significant correlation was also observed between the number of lymphatic vessels and blood vessels around the tumor, and between the number of lymphatic vessels and blood vessels inside the tumor. The investigations indicated a significant and direct correlation between lymphatic angiogenesis and blood angiogenesis (P-value < 0.05). Conclusion: Considering the above-mentioned results, it may be concluded that the procedure of lymphangiogenesis is more common than angiogenesis in melanoma. On the whole, both lymphangiogenesis and angiogenesis are more common in oral melanoma than dermal melanoma. We may also conclude that there is a significant and direct correlation between lymphangiogenesis and angiogenesis. © 2018 Medknow Publications. All rights reserved.