Thiopurine S-Methyltransferase Assay by Hplc in Acute Lymphoblastic Leukemia Patients and a Healthy Iranian Population

Thiopurine S-Methyltransferase Assay by Hplc in Acute Lymphoblastic Leukemia Patients and a Healthy Iranian Population

Mozafari S¹ ; Einollahi N² ; Shahgholi E³ ; Vardasbi S¹ ; Golestani A¹

Show Affiliations

1. Department of Clinical Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
2. Department of Medical Laboratory Sciences, Allied Medical Sciences School, Tehran University of Medical Sciences, Tehran, Iran
3. Department of Pediatrics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Acta Medica Iranica Published:2018

Abstract

Thiopurine s-methyltransferase (TPMT) plays a key role in the metabolism of the drug; 6-MP in children with acute lymphoblastic leukemia (ALL). The wild-type TPMT*1 allele and mutant alleles are associated with normal and intermediate enzyme activity, respectively. In patients with intermediate or deficient TPMT activity, normal dose of the drug may cause serious side effects such as bone marrow toxicity. The aim of this study was to assay the enzyme activity by HPLC for right ordering of chemotherapy drug doses in the patients. TPMT activity was measured in RBC of healthy adults (n=73) and children (n=10). Also, TPMT (*2, *3A, *3B and *3C) genotype of the samples were assessed by ARMS-PCR and RFLP-PCR. No indication of gender and age differences in the TPMT activity was found. This study showed that the HPLC method was sensitive, accurate and precise and can be used in routine clinical laboratory tests. © 2018 Tehran University of Medical Sciences. All rights reserved.

Style	Citing Format
MLA	Mozafari S, et al.. "Thiopurine S-Methyltransferase Assay by Hplc in Acute Lymphoblastic Leukemia Patients and a Healthy Iranian Population." Acta Medica Iranica, vol. 56, no. 5, 2018, pp. 301-307.
APA	Mozafari S, Einollahi N, Shahgholi E, Vardasbi S, Golestani A (2018). Thiopurine S-Methyltransferase Assay by Hplc in Acute Lymphoblastic Leukemia Patients and a Healthy Iranian Population. Acta Medica Iranica, 56(5), 301-307.
Chicago	Mozafari S, Einollahi N, Shahgholi E, Vardasbi S, Golestani A. "Thiopurine S-Methyltransferase Assay by Hplc in Acute Lymphoblastic Leukemia Patients and a Healthy Iranian Population." Acta Medica Iranica 56, no. 5 (2018): 301-307.
Harvard	Mozafari S et al. (2018) 'Thiopurine S-Methyltransferase Assay by Hplc in Acute Lymphoblastic Leukemia Patients and a Healthy Iranian Population', Acta Medica Iranica, 56(5), pp. 301-307.
Vancouver	Mozafari S, Einollahi N, Shahgholi E, Vardasbi S, Golestani A. Thiopurine S-Methyltransferase Assay by Hplc in Acute Lymphoblastic Leukemia Patients and a Healthy Iranian Population. Acta Medica Iranica. 2018;56(5):301-307.
BibTex	@article{ author = {Mozafari S and Einollahi N and Shahgholi E and Vardasbi S and Golestani A}, title = {Thiopurine S-Methyltransferase Assay by Hplc in Acute Lymphoblastic Leukemia Patients and a Healthy Iranian Population}, journal = {Acta Medica Iranica}, volume = {56}, number = {5}, pages = {301-307}, year = {2018} }
RIS	TY - JOUR AU - Mozafari S AU - Einollahi N AU - Shahgholi E AU - Vardasbi S AU - Golestani A TI - Thiopurine S-Methyltransferase Assay by Hplc in Acute Lymphoblastic Leukemia Patients and a Healthy Iranian Population JO - Acta Medica Iranica VL - 56 IS - 5 SP - 301 EP - 307 PY - 2018 ER -

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