Effect of Passage Number and Culture Time on the Expression and Activity of Insulin-Degrading Enzyme in Caco-2 Cells

Effect of Passage Number and Culture Time on the Expression and Activity of Insulin-Degrading Enzyme in Caco-2 Cells Publisher Pubmed

Mohammadi Farsani T¹ ; Motevaseli E² ; Neyazi N³ ; Khorramizadeh MR⁴ ; Zafarvahedian E⁵ ; Ghahremani MH^{2, 5}

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1. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
2. Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
3. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, International Campus (TUMS-IC), 88 Italia St, Tehran, Iran
4. Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
5. Department of Pharmacology and Toxicology, Faculty of pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Source: Iranian Biomedical Journal Published:2018

Abstract

Background: Insulin-degrading enzyme (IDE) is a conserved zinc metallopeptidase. Here, we have evaluated the effect of passage number and culture time on IDE expression and activity in colorectal adenocarcinoma cell line (Caco-2). Methods: Caco-2 cells were cultured with different passage ranges of 5-15, 25-35, 52-63 for 48, 72, and 120 hours. Subsequently, IDE expression and enzyme activity were assessed by Western blot analysis and fluorescent assay, respectively. Results: Our results confirmed that the amount of IDE was higher in cell extract compared to supernatant, and different passage numbers and culture times had small effect on IDE expression. However, when cells were cultured in the passage number range of 5-15 for 72 hours, the IDE activity was 35% higher compared to other passage numbers (p < 0.05). Conclusion: The use of Caco-2 cells at passage number range of 5-15 and culture time of 72 hours provides proper conditions for IDE-related studies. © 2018, Pasteur Institute of Iran. All rights reserved.

Style	Citing Format
MLA	Mohammadi Farsani T, et al.. "Effect of Passage Number and Culture Time on the Expression and Activity of Insulin-Degrading Enzyme in Caco-2 Cells." Iranian Biomedical Journal, vol. 22, no. 1, 2018, pp. 70-75.
APA	Mohammadi Farsani T, Motevaseli E, Neyazi N, Khorramizadeh MR, Zafarvahedian E, Ghahremani MH (2018). Effect of Passage Number and Culture Time on the Expression and Activity of Insulin-Degrading Enzyme in Caco-2 Cells. Iranian Biomedical Journal, 22(1), 70-75.
Chicago	Mohammadi Farsani T, Motevaseli E, Neyazi N, Khorramizadeh MR, Zafarvahedian E, Ghahremani MH. "Effect of Passage Number and Culture Time on the Expression and Activity of Insulin-Degrading Enzyme in Caco-2 Cells." Iranian Biomedical Journal 22, no. 1 (2018): 70-75.
Harvard	Mohammadi Farsani T et al. (2018) 'Effect of Passage Number and Culture Time on the Expression and Activity of Insulin-Degrading Enzyme in Caco-2 Cells', Iranian Biomedical Journal, 22(1), pp. 70-75.
Vancouver	Mohammadi Farsani T, Motevaseli E, Neyazi N, Khorramizadeh MR, Zafarvahedian E, Ghahremani MH. Effect of Passage Number and Culture Time on the Expression and Activity of Insulin-Degrading Enzyme in Caco-2 Cells. Iranian Biomedical Journal. 2018;22(1):70-75.
BibTex	@article{ author = {Mohammadi Farsani T and Motevaseli E and Neyazi N and Khorramizadeh MR and Zafarvahedian E and Ghahremani MH}, title = {Effect of Passage Number and Culture Time on the Expression and Activity of Insulin-Degrading Enzyme in Caco-2 Cells}, journal = {Iranian Biomedical Journal}, volume = {22}, number = {1}, pages = {70-75}, year = {2018} }
RIS	TY - JOUR AU - Mohammadi Farsani T AU - Motevaseli E AU - Neyazi N AU - Khorramizadeh MR AU - Zafarvahedian E AU - Ghahremani MH TI - Effect of Passage Number and Culture Time on the Expression and Activity of Insulin-Degrading Enzyme in Caco-2 Cells JO - Iranian Biomedical Journal VL - 22 IS - 1 SP - 70 EP - 75 PY - 2018 ER -

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