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Production of Bacteriocin by Various Strains of Pediococcus Acidilactiduring Batch Fermentation and Identification of the Pediocin Structural Genes Publisher



Ahmadianmoghadam H1, 3, 5 ; Vafadoost R2 ; Elegado FB1, 3 ; Aghajani D4 ; Perez MTM3
Authors
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Authors Affiliations
  1. 1. Institute of Biological Science, College of Science, University of the Philippines Los Banos, Laguna, Philippines
  2. 2. School of Medicine, Islamic Azad University of Medical Science, Tehran, Iran
  3. 3. National Institute of Molecular biology and Biotechnology, University of the Philippines Los Banos, Laguna, Philippines
  4. 4. School of Medicine, Shahid Beheshti University of Medical Science, Tehran, Iran
  5. 5. Department of Genetics, Iranian Institute for Reduction of High-Risk Behaviors, Tehran University of Medical Sciences, Tehran, Iran

Source: Biotekhnologiya Published:2021


Abstract

Bacteriocins are antimicrobial peptides produced by certain bacteria that can be alternatives to traditional antibiotics. This study aimed at evaluating large-scale bacteriocin production by the Pediococcus acidilactici strains in batch fermentation and to analyze the pediocin structural gene (papA) by bioinformatic methods. The fermentation using bacterial strains was carried out in Sartorius Biostat A-Plus Bioreactor, and the bacteriocin production was tested on Listeria innocua as a result of 24 h fermentation. The pediocin structural gene papA was amplified, and the amplicons of each strain were sequenced and analyzed to assess the secondary structure of pediocin and related metabolic pathways. It was shown that the papA structural gene sequence is a conserved region. All strains with a papA amplicon synthesis exhibited active bacteriocin synthesis. © 2021, State Research Institute for Genetics and Selection of Industrial Microorganisms. All rights reserved.