Tehran University of Medical Sciences

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Comparison of Gold Nanoparticle Conjugated Secondary Antibody With Non-Gold Secondary Antibody in an Elisa Kit Model Publisher Pubmed



Emami T1, 3 ; Madani R1 ; Golchinfar F1 ; Shoushtary A2 ; Amini SM3
Authors

Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Published:2015


Abstract

In this study, gold nanoparticles (AuNPs) were used as carriers of the signaling anti-chicken antibody peroxidase in comparison with anti-chicken antibody peroxidase without gold nanoparticle in a commercial avian influenza kit. AuNPs enhanced the absorbance and shortened the assay time. AuNPs act as a carrier of many enzymes and multiply the effect of enzyme when reacting with substrate. They amplify optical signal, while keeping low background signals. © 2015, Mary Ann Liebert, Inc.