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Variation of Housekeeping Genes in Clinical Isolates and Vaccine Strains of Bordetella Pertussis Publisher Pubmed



Fathi M1 ; Haghighi F1 ; Shahcheraghi F2 ; Abbasi E3 ; Eshraghi SS1 ; Ghourchian S1 ; Zeraati H4 ; Yaseri M4 ; Douraghi M1, 5 ; Shokri F6, 7
Authors
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Authors Affiliations
  1. 1. Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, P.O. Box 14155-6446, Tehran, Iran
  2. 2. Department of Bacteriology and Microbiology, Pasteur Institute of Iran, Tehran, Iran
  3. 3. Department of Bacterial Vaccines, Razi Vaccine and Serum Research Institute, Karaj, Iran
  4. 4. Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Department of Immunology, School of Public Health, Tehran University of Medical Sciences, P.O. Box: 14155-1664, Tehran, Iran
  7. 7. Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran

Source: Clinical Laboratory Published:2017


Abstract

Background: Bordetella pertussis causes serious contagious infections, primarily in childhood. A whole-cell vaccine, diphtheria-tetanus-whole cell pertussis (DTwP), has been used to protect against pertussis in children in Iran, but the pertussis cases have been increasing during recent years. We determined the allelic variation level of housekeeping genes in isolates recovered from pertussis patients and vaccine strains used in national vaccination program. Methods: Five clinical isolates, 2 vaccine strains and a Tohama I strain were studied through multilocus sequence typing (MLST) of housekeeping genes. The relatedness between STs, the founder, single-and double-locus variants (SLVs, DLVs) was determined using eBURST algorithm. The concordance between the type assignments by MLST and PFGE was determined. Results: In the 5 clinical isolates, 2 STs were identified, ST2 and ST79. The vaccine strains displayed two distinct allelic profiles assigned to STI and ST2. ST2 was predicted as founder and the remaining STs were SLVs of ST2. MLST and PFGE type assignments were 86.6% concordant. Conclusions: The clinical isolates of B. pertussis were different from vaccine strains used in the national vaccination program. This study confirms the low level of variation in housekeeping genes of B. pertussis. MLST of virulent antigenic genes needs to be applied as a complementary method for the characterization of new ST-harboring isolates that may predominate periodically. The combination of these data allows rapid and efficient surveillance of currently circulating isolates. These data might elucidate the future trends and considerations for vaccine formulation and design.
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