Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Disturbed Transcription of Tlrs’ Negative Regulators and Cytokines Secretion Among Tlr4- and 9-Activated Pbmcs of Agammaglobulinemic Patients Publisher Pubmed



Sanaei R1, 2 ; Rezaei N2, 3, 4 ; Aghamohammadi A3 ; Delbandi AA1, 5 ; Tavasolian P3 ; Tajik N1, 5
Authors
Show Affiliations
Authors Affiliations
  1. 1. Immunology Research Center (IRC), Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran
  2. 2. Network of Immunity in Infection, Malignancy and Autoimmunity (NIIMA), Universal Scientific Education and Research Network (USERN), Tehran, Iran
  3. 3. Research Center for Immunodeficiencies, Children’s Medical Center, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Primary Immunodeficiency Diseases Network (PIDNet), Universal Scientific Education and Research Network (USERN), Tehran, Iran
  5. 5. Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

Source: Immunological Investigations Published:2019


Abstract

Toll-like receptors (TLRs) are inevitable elements for immunity development and antibody production. TLRs are in close interaction with Bruton’s tyrosine kinase which has been found mutated and malfunctioned in the prototype antibody deficiency disease named X-linked agammaglobulinemia (XLA). TLRs’ ability was evaluated to induce transcription of TLR-negative regulators, including suppressor of cytokine signaling 1 (SOCS1), interleukin-1 receptor-associated kinase 3 (IRAK-M), tumor necrosis factor alpha-induced protein 3 (TNFAIP3, A20), and Ring finger protein 216 (RNF216), and Tumor necrosis factor-α (TNF-α) and Interferon-α (IFN-α) production via Lipopolysaccharides (LPS) and CpG-A oligodeoxynucleotides (CpG-A ODN). Measured by TaqMan real-time polymerase chain reaction (PCR), meaningfully increased transcripts of SOCS1 and RNF216 were found in XLA peripheral blood mononuclear cells (PBMCs). Also, TLR inductions of XLA have led to similar downregulations in the regulator’s transcription which was different from that in healthy donors. Cytokine measurement by enzyme-linked immunosorbent assay (ELISA) revealed a significant lower TNF-α production both before and after LPS. By selected molecules in this study, TLRs’ potential defectiveness range expands TLRs expression, downstream signaling, and cytokine production. The results show new potential elements that could play a part in TLRs defect and pathogenesis of agammaglobulinemia as well. © 2019, © 2019 Taylor & Francis Group, LLC.
Other Related Docs
9. Intravenous Immunoglobulin Therapy in Myocarditis, Iranian Journal of Allergy# Asthma and Immunology (2020)
10. Autoimmunity in Primary Antibody Deficiencies, International Archives of Allergy and Immunology (2017)
11. Primary Immunodeficiencies and Cancers, Cancer Immunology: A Translational Medicine Context (2015)
13. Inborn Errors of Immunity and Cancers, Cancer Immunology: A Translational Medicine Context# Second Edition (2020)