Effect of Gamma Irradiation on Cytotoxicity, Phenolics Content and Acute Toxicity of Cuscuta Chinensis L. Extract Publisher



Alijaniha F^{1, 2} ; Emadi F^{1, 2} ; Naseri M^{1, 2} ; Kamalinejad M³ ; Motevaseli E⁴ ; Soodi M⁵ ; Karimi R⁶ Show Affiliations
Source: Radiation Physics and Chemistry Published:2021

Abstract

Gamma irradiation is used as an efficient method for decontamination of some foodstuffs and herbal medicines. Several studies have shown that low to moderate doses of gamma irradiation do not alter the chemical composition and biological activities of herbal medicine; however, some changes in herbal composition, appearance or properties have been observed in another studies, and this remains controversial. The present study aimed to evaluate the effect of gamma irradiation on the biological activity and the content of bioactive compounds of Cuscuta chinensis L. extract. The dose of 5 kGy was studied for decontamination. The results of the in vitro study showed that the cytotoxic effects on A549 and HT-29 cell lines were improved without any alteration of cytotoxicity on normal cell line. Also, acute oral toxicity of the extract did not change according to in vivo study. However, due to the significant reduction of total phenol and flavonoids contents by 12% and 18% respectively, the improvement of cytotoxic properties maybe due to the formation of new active compounds, the identification of which requires further studies. © 2021 Elsevier Ltd