A Highly Specific and Sensitive Loop-Mediated Isothermal Amplification Method for the Detection of Escherichia Coli O157: H7

A Highly Specific and Sensitive Loop-Mediated Isothermal Amplification Method for the Detection of Escherichia Coli O157: H7 Publisher Pubmed

Ravan H¹ ; Amandadi M¹ ; Sanadgol N^{2, 3}

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1. Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, Iran
2. Department of Biology, Faculty of Science, Zabol University, Zabol, Iran
3. Pharmaceutical Science Research Center (PSRC), Tehran University of Medical Sciences (TUMS), Tehran, Iran

Source: Microbial Pathogenesis Published:2016

Abstract

E. coli O157:H7 is one of the most important foodborne pathogen that causes some human illnesses such as bloody diarrhea, hemolytic-uremic syndrome, and kidney failure. We developed a loop-mediated isothermal amplification (LAMP) assay with six special primers that target a highly specific 299-bp region of the Z3276 gene for the detection of E. coli O157:H7. Among 117 bacterial strains tested in this study, positive results were only obtained from E. coli O157:H7 strains. The sensitivity level of the Z3276-LAMP assay was determined to be 5 CFU/reaction tube in pure bacterial culture. Moreover, the LAMP assay was successfully applied to artificially contaminated ground beef with a sensitivity level of 103 CFU/mL without pre-enrichment and 10 CFU/mL after a 4-h pre-enrichment. In conclusion, the present LAMP assay would be a useful and powerful tool for the rapid, sensitive, and specific diagnosis of E. coli O157:H7 strains in resource limited laboratories. © 2015 Elsevier Ltd.

2. Molecular Design Real Time Loop-Mediated Isothermal Amplification Method for Rapid Detection of Neisseria Meningitidis; [Метод Молекулярного Конструирования Петлевой Изотермической Амплификации В Реальном Времени Для Быстрого Обнаружения Neisseria Meningitidis], Folia Medica (2021)

Experts (# of related papers)

Solmaz Ohadian Moghadam (1)

Mohammad Reza Pourmand (1)

Style	Citing Format
MLA	Ravan H, Amandadi M, Sanadgol N. "A Highly Specific and Sensitive Loop-Mediated Isothermal Amplification Method for the Detection of Escherichia Coli O157: H7." Microbial Pathogenesis, vol. 91, no. , 2016, pp. 161-165.
APA	Ravan H, Amandadi M, Sanadgol N (2016). A Highly Specific and Sensitive Loop-Mediated Isothermal Amplification Method for the Detection of Escherichia Coli O157: H7. Microbial Pathogenesis, 91(), 161-165.
Chicago	Ravan H, Amandadi M, Sanadgol N. "A Highly Specific and Sensitive Loop-Mediated Isothermal Amplification Method for the Detection of Escherichia Coli O157: H7." Microbial Pathogenesis 91, no. (2016): 161-165.
Harvard	Ravan H, Amandadi M, Sanadgol N (2016) 'A Highly Specific and Sensitive Loop-Mediated Isothermal Amplification Method for the Detection of Escherichia Coli O157: H7', Microbial Pathogenesis, 91(), pp. 161-165.
Vancouver	Ravan H, Amandadi M, Sanadgol N. A Highly Specific and Sensitive Loop-Mediated Isothermal Amplification Method for the Detection of Escherichia Coli O157: H7. Microbial Pathogenesis. 2016;91():161-165.
BibTex	@article{ author = {Ravan H and Amandadi M and Sanadgol N}, title = {A Highly Specific and Sensitive Loop-Mediated Isothermal Amplification Method for the Detection of Escherichia Coli O157: H7}, journal = {Microbial Pathogenesis}, volume = {91}, number = {}, pages = {161-165}, year = {2016} }
RIS	TY - JOUR AU - Ravan H AU - Amandadi M AU - Sanadgol N TI - A Highly Specific and Sensitive Loop-Mediated Isothermal Amplification Method for the Detection of Escherichia Coli O157: H7 JO - Microbial Pathogenesis VL - 91 IS - SP - 161 EP - 165 PY - 2016 ER -

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