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A Bioassay-Guided Fractionation Scheme for Characterization of New Antibacterial Compounds From Prosopis Cineraria Aerial Parts



Neghabihajiagha M1 ; Aliahmadi A2 ; Taheri MR3 ; Ghassempour A3 ; Irajian G4 ; Rezadoost H3 ; Feizabadi MM1
Authors
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Authors Affiliations
  1. 1. Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Poorsina street, Tehran, Iran
  2. 2. Department of Biology, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Iran
  3. 3. Department of Phytochemistry, Medicinal Plants and Drug Research Institute, Shahid Beheshti University, Tehran, Iran
  4. 4. Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

Source: Iranian Journal of Microbiology Published:2016

Abstract

Background and Objectives:Due to the importance of finding of new antibacterial agents, the antibacterial properties of Prosopis cinerariaaerial parts were investigated using a bioassay guided fractionation scheme. Materials and Methods:The organic extract was prepared via maceration in methanol, followed by the fractionation using n-hexane and ethyl acetate. The MICs of fractions were determined against some human pathogenic bacteria using broth micro-dilution assay. The primary characterization and identification of bioactive substance(s) was based on a bio-autograph- ical method using HPTLC and flash chromatography in parallel with agar overlay assays. Finally the exact mass of effective compound(s) was determined by LC-MS. Results:The best antibacterial activities were related to the ethyl acetate fraction. The effective antibacterial compound of the plant were 2 substances with molecular weight of 348 and 184 Dalton that inhibited the growth of assessed Gram positive bacteria with MIC values lower than 125 to 62.5 µg/ml synergistically. Conclusion: Further analysis using nuclear magnetic resonance could reveal the exact structure of these two antibacterial substances. These 2 effective antibacterial compounds could be applied as lead compound for synthesis of new antibacterial agents. © 2016, Tehran University of Medical Science. All Rights reserved.