Up-Regulation of Mir-130B Expression Level and Down-Regulation of Mir-218 Serve As Potential Biomarker in the Early Detection of Human Osteosarcoma Publisher Pubmed



Taheriazam A¹ ; Talaei AJ² ; Jamshidi M^{3, 4} ; Shakeri M⁵ ; Khoshbakht S⁶ ; Yahaghi E⁷ ; Shokrani M⁸ Show Affiliations
Source: Diagnostic Pathology Published:2015

Abstract

Background: Osteosarcoma (OS) is a primary malignant bone tumor with high morbidity that principally emerges in children and adolescents. MiRNAs regulate a variety of normal physiologic processes and are involved in tumorigenesis and development of multiple malignancies, including OS. This study was aimed to evaluate the clinical significance of miR-130b and miR-218 in osteosarcoma patient. Methods: We utilized quantitative real-time PCR to evaluate the level of miR-130b and miR-218 expressions in OS patients and normal tissues and their relationship with clinicopathological features and survival in OS patients. Results: QRT-PCR indicated that miR-130b expression in tumor tissues was strongly elevated than adjacent non-tumor tissues (P < 0.001), while the level of miR-218 expression in osteosarcoma tissues was down-regulated than adjacent non-tumor tissues (P < 0.001). We evaluated the clinical significance of miR-130b and miR-218 in osteosarcoma. Clinical correlation analysis showed that increased expression of miR-130b and decreased expression of miR-218 were significantly associated with advanced tumor stage (x 2 = 6.285, P < 0.009; x 2 = 7.172, P < 0.007), distant metastasis (x 2 = 5.528; P < 0.001; x 2 = 4.617, P < 0.001) and size of tumor (x 2 = 5.01, P = 0.013; x 2 = 4.271, P = 0.019). Conclusions: Taken together, our data indicated that high miR-130b level and low level of miR-218 are associated with poor clinicopathological characteristics. Furthermore, miR-130b may play a key role in the progression of osteosarcoma. © 2015 Taheriazam et al.