In Vitro Assay of Paecilomyces Lilacinus Biocontrol Effects on Fasciola Hepatica Eggs Illustrated in Scanning Electron Micrographs



Najafi F¹ ; Rezaie S¹ ; Kia EB¹ ; Mahmoudi M² ; Khodavaisy S¹ ; Mohebali M^{1, 3} ; Gharagozlou MJ⁴ ; Rokni MB¹ ; Mowlavi G^{1, 4} Show Affiliations
Source: Iranian Journal of Parasitology Published:2017

Abstract

Background: Fascioliasis is a zoonotic disease caused by the liver fluke Fasciola hepatica. Drug resistance, high costs of treatment and economic losses in meat production have emerged the need of alternative control measures into consideration. The aim of this study was to evaluate the in vitro ovicidal activity of Paecilomyces lilacinus fungus on F. hepatica eggs. Methods: P. lilacinus isolated from the soil of natural environment was challenged on F. hepatica eggs to observe the bio control effect of nematophagous fungi on trematode helminth eggs. The study was conducted in Tehran University of Medical Sciences, in 2015. Within 21 d of experiment, destructive effects exhibited on the eggshells were investigated using optical and Scanning Electron Microscopy. Results: The effective role of P. lilacinus on damaging the eggs of F. hepatica was noticed. Conclusion: This finding is promising for advantageous use of nematophagus fungi as a natural constituent in hyper endemic areas for certain helminthic infections like fascioliasis with diverse kinds of herbivores as egg passer hosts. © 2017, Tehran University of Medical Sciences (TUMS). All rights reserved.