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Development and Validation of an Analytical Rp-Hplc Method for Simultaneous Estimation of Losartan and Its Active Metabolite (Exp-3174) in Isolated Perfused Rat Liver Publisher



Toolabi M1 ; Ramezankhani R1 ; Rahbar N1, 3 ; Dibaei M4 ; Foroumadi A5 ; Lavasani H2 ; Kazemi V6 ; Rouini M2
Authors
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Authors Affiliations
  1. 1. Department of Medicinal Chemistry, Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
  2. 2. Biopharmaceutics and Pharmacokinetic Division, Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Nanotechnology Research Center, Faculty of Pharmacy, Ahvaz Jun-dishapur University of Medical Sciences, Ahvaz, Iran
  4. 4. Razi Drug Research Center, Iran University of Medical Scienc-es, Tehran, Iran
  5. 5. Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Food and Drug Laboratory Research Center, Food and Drug Administration, MOH & ME, Tehran, Iran

Source: Current Pharmaceutical Analysis Published:2023


Abstract

Background: The liver perfusion method is frequently used in drug pharmacokinetic studies and the various effects of drugs on liver tissue. The aim of this study was to establish and validate an analytical method using high-performance liquid chromatography to determine the simultaneous concentration of losartan and its active metabolite, EXP-3174, in an isolated perfused rat liver study. Method: An HPLC system with isocratic mode was used. Various chromatographic parameters were adjusted to develop and validate a method for determination of losartan and its active metabolite in liver perfusion media. Results: In this study, losartan and its active metabolite, EXP-3174, were separated using a C18 stationary phase, a mobile phase consisting of acetonitrile: phosphate buffer at a flow rate of 1 mL.min-1, and UV detection at 254 nm. Retention times for losartan and the metabolite were 10 and 16 minutes, respectively. Linearity from 25-250 ng.ml-1 was validated with acceptable accuracy and precision. The LOD and LOQ for losartan were 7.0 and 21.1 ng.ml-1, respectively. The LOD and LOQ for metabolite were 7.4 and 22.4 ng.ml-1, respectively. ChromGate® software was used to acquire and process the data. Conclusions: The optimized and validated technique was effectively used to analyze losartan and its active metabolite in isolated perfused rat liver. © 2023 Bentham Science Publishers.
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