Construction of Genetically Engineered M13k07 Helper Phage for Simultaneous Phage Display of Gold Binding Peptide 1 and Nuclear Matrix Protein 22 Scfv Antibody

Construction of Genetically Engineered M13k07 Helper Phage for Simultaneous Phage Display of Gold Binding Peptide 1 and Nuclear Matrix Protein 22 Scfv Antibody Publisher Pubmed

Fatemi F¹ ; Amini SM^{2, 3} ; Kharrazi S² ; Rasaee MJ⁴ ; Mazlomi MA¹ ; Asadighalehni M¹ ; Rajabibazl M^{5, 6} ; Sadroddiny E¹

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1. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
2. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
3. Radiation Biology Research Center, Iran University of Medical Sciences, Tehran, Iran
4. Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
5. Department of Clinical Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
6. School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Colloids and Surfaces B: Biointerfaces Published:2017

Abstract

The most common techniques of antibody phage display are based on the use of M13 filamentous bacteriophages. This study introduces a new genetically engineered M13K07 helper phage displaying multiple copies of a known gold binding peptide on p8 coat proteins. The recombinant helper phages were used to rescue a phagemid vector encoding the p3 coat protein fused to the nuclear matrix protein 22 (NMP22) ScFv antibody. Transmission electron microscopy (TEM), UV–vis absorbance spectroscopy, and field emission scanning electron microscopy (FE-SEM) with energy dispersive X-ray spectroscopy (EDX) analysis revealed that the expression of gold binding peptide 1 (GBP1) on major coat protein p8 significantly enhances the gold-binding affinity of M13 phages. The recombinant bacteriophages at concentrations above 5 × 104 pfu/ml red-shifted the UV–vis absorbance spectra of gold nanoparticles (AuNPs); however, the surface plasmon resonance of gold nanoparticles was not changed by the wild type bacteriophages at concentrations up to 1012 pfu/ml. The phage ELISA assay demonstrated the high affinity binding of bifunctional bacteriophages to NMP22 antigen at concentrations of 105 and 106 pfu/ml. Thus, the p3 end of the bifunctional bacteriophages would be able to bind to specific target antigen, while the AuNPs were assembled along the coat of virus for signal generation. Our results indicated that the complex of antigen-bacteriophages lead to UV–vis spectral changes of AuNPs and NMP22 antigen in concentration range of 10–80 μg/ml can be detected by bifunctional bacteriophages at concentration of 104 pfu/ml. The ability of bifunctional bacteriophages to bind to antigen and generate signal at the same time, makes this approach applicable for identifying different antigens in immunoassay techniques. © 2017 Elsevier B.V.

Style	Citing Format
MLA	Fatemi F, et al.. "Construction of Genetically Engineered M13k07 Helper Phage for Simultaneous Phage Display of Gold Binding Peptide 1 and Nuclear Matrix Protein 22 Scfv Antibody." Colloids and Surfaces B: Biointerfaces, vol. 159, no. , 2017, pp. 770-780.
APA	Fatemi F, Amini SM, Kharrazi S, Rasaee MJ, Mazlomi MA, Asadighalehni M, Rajabibazl M, Sadroddiny E (2017). Construction of Genetically Engineered M13k07 Helper Phage for Simultaneous Phage Display of Gold Binding Peptide 1 and Nuclear Matrix Protein 22 Scfv Antibody. Colloids and Surfaces B: Biointerfaces, 159(), 770-780.
Chicago	Fatemi F, Amini SM, Kharrazi S, Rasaee MJ, Mazlomi MA, Asadighalehni M, Rajabibazl M, Sadroddiny E. "Construction of Genetically Engineered M13k07 Helper Phage for Simultaneous Phage Display of Gold Binding Peptide 1 and Nuclear Matrix Protein 22 Scfv Antibody." Colloids and Surfaces B: Biointerfaces 159, no. (2017): 770-780.
Harvard	Fatemi F et al. (2017) 'Construction of Genetically Engineered M13k07 Helper Phage for Simultaneous Phage Display of Gold Binding Peptide 1 and Nuclear Matrix Protein 22 Scfv Antibody', Colloids and Surfaces B: Biointerfaces, 159(), pp. 770-780.
Vancouver	Fatemi F, Amini SM, Kharrazi S, Rasaee MJ, Mazlomi MA, Asadighalehni M, et al.. Construction of Genetically Engineered M13k07 Helper Phage for Simultaneous Phage Display of Gold Binding Peptide 1 and Nuclear Matrix Protein 22 Scfv Antibody. Colloids and Surfaces B: Biointerfaces. 2017;159():770-780.
BibTex	@article{ author = {Fatemi F and Amini SM and Kharrazi S and Rasaee MJ and Mazlomi MA and Asadighalehni M and Rajabibazl M and Sadroddiny E}, title = {Construction of Genetically Engineered M13k07 Helper Phage for Simultaneous Phage Display of Gold Binding Peptide 1 and Nuclear Matrix Protein 22 Scfv Antibody}, journal = {Colloids and Surfaces B: Biointerfaces}, volume = {159}, number = {}, pages = {770-780}, year = {2017} }
RIS	TY - JOUR AU - Fatemi F AU - Amini SM AU - Kharrazi S AU - Rasaee MJ AU - Mazlomi MA AU - Asadighalehni M AU - Rajabibazl M AU - Sadroddiny E TI - Construction of Genetically Engineered M13k07 Helper Phage for Simultaneous Phage Display of Gold Binding Peptide 1 and Nuclear Matrix Protein 22 Scfv Antibody JO - Colloids and Surfaces B: Biointerfaces VL - 159 IS - SP - 770 EP - 780 PY - 2017 ER -

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