Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Degradation of Sesame Oil Phenolics Using Magnetic Immobilized Laccase Publisher



Amin R1 ; Khorshidi A1 ; Bensch W2 ; Senkale S2 ; Faramarzi MA3
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Chemistry, Faculty of Sciences, University of Guilan, P.O. Box 41335-1914, Rasht, Guilan, Iran
  2. 2. Institute of Inorganic Chemistry, Kiel University, Max-Eyth Straβe 2, Kiel, 24118, Germany
  3. 3. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155–6451, Tehran, 1417614411, Iran

Source: Catalysis Letters Published:2020


Abstract

Abstract: Phenolic compounds exhibit toxic effects and there are still many challenges to find an efficient way for degradation and removal of phenol especially from food. In the present study, magnetically immobilized laccase was prepared and applied as an efficient heterogeneous biocatalyst for biodegradation of phenol from sesame oil. Laccase was attached covalently to magnetic Fe3O4 nanospheres and nanorods, and the characteristics of the immobilized enzyme were studied. The magnetic supports were analyzed by scanning electron microscopy, X-ray diffraction, vibrating sample magnetometry, and Fourier-transform infrared spectroscopy. Storage stability analysis of immobilized enzyme showed that more than 70% of initial activity was kept after 15 days at 4 °C. More than 70% phenol degradation was achieved and a 60% decrease in the activity of immobilized laccase was observed after 20 independent cycles. The development of enzyme immobilization techniques on magnetic supports may expand the potential applications of heterogeneous biocatalysts in food industries. Graphic Abstract: [Figure not available: see fulltext.]. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.