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Impact of Preconditioned Diabetic Stem Cells and Photobiomodulation on Quantity and Degranulation of Mast Cells in a Delayed Healing Wound Simulation in Type One Diabetic Rats Publisher Pubmed



Ahmadi H1 ; Bayat M1, 9 ; Amini A1 ; Mostafavinia A2 ; Ebrahimpourmalekshah R1 ; Gazor R3 ; Asadi R4 ; Gachkar L5 ; Rezaei F6 ; Shafikhani SH7 ; Ghoreishi SK8 ; Chien S9
Authors
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Authors Affiliations
  1. 1. Department of Biology and Anatomical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  2. 2. Department of Anatomy, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
  3. 3. Department of Anatomy, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
  4. 4. Paramedical School, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Infectious Diseases Department, Loghman Hakim Hospital, Kamali St., South Karegar St., Tehran, Iran
  6. 6. College of Pharmacy, University of Kentucky, 789 South Limestone, Lexington, 40536, KY, United States
  7. 7. Department of Medicine, Division of Hematology/Oncology/Cell Therapy, Department of Microbial Pathogens and Immunity, Cancer Center, Rush University Medical Center, Chicago, IL, United States
  8. 8. Department of Statistics, Qom University, Qom, Iran
  9. 9. Price Institute of Surgical Research, University of Louisville, and Noveratech LLC, Louisville, KY, United States

Source: Lasers in Medical Science Published:2022


Abstract

Herein, we report the influence of administering different protocols of preconditioned diabetic adipose-derived mesenchymal stem cells (ADSs) with photobiomodulation in vitro, and photobiomodulation in vivo on the number of mast cells (MCs), their degranulation, and wound strength in the maturation step of a Methicillin-resistant Staphylococcus aureus (MRSA)-infectious wound model in rats with type one diabetes. An MRSA-infectious wound model was generated on diabetic animals, and they were arbitrarily assigned into five groups (G). G1 were control rats. In G2, diabetic ADS were engrafted into the wounds. In G3, diabetic ADS were engrafted into the wound, and the wound was exposed to photobiomodulation (890 nm, 890 ± 10 nm, 80 Hz, 0.2 J/cm2) in vivo. In G4, preconditioned diabetic ADS with photobiomodulation (630 and 810 nm; each 3 times with 1.2 J/cm2) in vitro were engrafted into the wound. In G5, preconditioned diabetic ADS with photobiomodulation were engrafted into the wound, and the wound was exposed to photobiomodulation in vivo. The results showed that, the maximum force in all treatment groups was remarkably greater compared to the control group (all, p = 0.000). Maximum force in G4 and G5 were superior than that other treated groups (both p = 0.000). Moreover, G3, G4, and G5 showed remarkable decreases in completely released MC granules and total numbers of MC compared to G1 and G2 (all, p = 0.000). We concluded that diabetic rats in group 5 showed significantly better results in terms of accelerated wound healing and MC count of an ischemic infected delayed healing wound model. © 2021, The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature.
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