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Immunovirological Assessment of Hiv-Infected Patients and Phylogenetic Analysis of the Virus in Northeast of Iran Publisher



Abbasnia S1 ; Hassannia T2 ; Ariaee N1 ; Sabet F1 ; Pashabayg KR3 ; Keyvani H4 ; Sarvghad MR5 ; Ziaeemehr A1 ; Fakor F1 ; Valizadeh N1
Authors
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Authors Affiliations
  1. 1. Immunology Research Center, Inflammation and Inflammatory Diseases Division, Mashhad University of Medical Sciences, Mashhad, Iran
  2. 2. Internal Medicine Department, Arash Hospital, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Virology, Iran University of Medical Sciences, Tehran, Iran
  5. 5. Internal Medicine Department, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran

Source: Jundishapur Journal of Microbiology Published:2021


Abstract

Background: The reliable laboratory tests, co-infection with other tumor viruses, and determining the genetic types are very important for therapy and monitoring of the clinical status of human immunodeficiency virus (HIV)-infected subjects. Objectives: This study evaluated the co-infection of HIV with hepatitis B virus (HBV), hepatitis C virus (HCV), and human T-cell leukemia virus type 1 (HTLV-1), the viral load, the progression of infection, and its correlation with the clinical status. Methods: Twenty HIV-infected cases were assessed for T cell subpopulations, HBV, HCV, and HTLV-1 co-infection, as well as the viral load. For phylogenetic relationships analysis, the HIV-c2-v5 fragment and p17 of gag were amplified, sequenced, and then clustered using phylogenetic analysis by MEGA software with maximum-likelihood. Results: The quantity of HIV viral load by qRT-PCR (TaqMan) and Cobas-Amplicor monitor test had a very strong correlation (R = 0.881, P < 0.0001). A significant negative correlation was also found between CD4+ cell count and Cobas-Amplicor (R =-0.41, P = 0.06). A significant negative correlation was also found between CD4+ cell count and Cobas-Amplicor (R =-0.41, P = 0.06) with HIV monitor test results (R =-0.41, P = 0.06).. The phylogenetic analysis for p17 regions in gag and c2-v5 in env genes showed that all subjects had AD genotype. The co-infection of the HIV subjects with HBV, HCV, and HTLV-1 was 75%, 75%, and 15%, respectively. A direct correlation was observed between CD8+ and HIV-HTLV-1 co-infection. Conclusions: The results showed that HIV CRF35-AD, (M group) is more frequent in the northeast of Iran, and both real-time quantification methods were reliable for monitoring the HIV-1 viral load. In addition, the transmission rate of HTLV-1 is lower than HBV and HCV among drug abusers. © 2021, Author(s).
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