The Osmolyte Type Affects Cartilage Associated Pathologic Marker Expression During in Vitro Mesenchymal Stem Cell Chondrogenesis Under Hypertonic Conditions

The Osmolyte Type Affects Cartilage Associated Pathologic Marker Expression During in Vitro Mesenchymal Stem Cell Chondrogenesis Under Hypertonic Conditions Publisher Pubmed

Ahmadyan S^{1, 2} ; Kabiri M¹ ; Tasharofi N^{2, 3} ; Hosseinzadeh S⁴ ; Kehtari M⁵ ; Zadeh AH² ; Soleimani M⁶ ; Farazmand A⁵ ; Hanaeeahvaz H²

Show Affiliations

1. Department of Biotechnology, College of science, University of Tehran, Tehran, Iran
2. Stem Cell Technology Research Center, Tehran, Iran
3. Department of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
4. School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5. Faculty of Biology, University of Tehran, Tehran, Iran
6. Department of Hematology, Tarbiat Modares University, Tehran, Iran

Source: Cellular and Molecular Biology Published:2018

Abstract

Stem cells' fate during in vitro differentiation is influenced by biophysicochemical cues. Osmotic stress has proved to enhance chondrocyte marker expression, however its potent negative impacts had never been surveyed. We questioned whether specific osmotic conditions, regarding the osmolyte agent, could benefit chondrogenesis while dampening undesired concomitant hypertrophy and inflammatory responses. To examine the potential side effects of hypertonicity, we assessed cell proliferation as well as chondrogenic and hypertrophic marker expression of human Adipose Derived-MSC after a two week induction in chondrogenic media with either NaCl or Sorbitol, as the osmolyte agent to reach a +100 mOsm hypertonic condition. Calcium deposition and TNF-α secretion as markers associated with hypertrophy and inflammation were then assayed. While both hyperosmotic conditions upregulated chondrogenic markers, sorbitol had a nearly three times higher chondro-promotive effect and a lesser hypertrophic effect compared to NaCl. Also, a significantly lesser calcium deposition was observed in sorbitol hypertonic group. NaCl showed an anti-proinflammatory effect while sorbitol had no effect on inflammatory markers. The ossification potential and cartilage associated pathologic markers were affected differentially by the type of the osmolyte. Thus, a vigilant application of the osmotic agent is inevitable in order to avoid or reduce undesired hypertrophic and inflammatory phenotype acquisition by MSC during chondrogenic differentiation. Our findings are a step towards developing a more reliable chondrogenic regimen using external hypertonic cues for MSC chondrogenesis with potential applications in chondral lesions cell therapy. © 2018 by the C.M.B. Association.

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Style	Citing Format
MLA	Ahmadyan S, et al.. "The Osmolyte Type Affects Cartilage Associated Pathologic Marker Expression During in Vitro Mesenchymal Stem Cell Chondrogenesis Under Hypertonic Conditions." Cellular and Molecular Biology, vol. 6, no. 3, 2018, pp. 56-61.
APA	Ahmadyan S, Kabiri M, Tasharofi N, Hosseinzadeh S, Kehtari M, Zadeh AH, Soleimani M, Farazmand A, Hanaeeahvaz H (2018). The Osmolyte Type Affects Cartilage Associated Pathologic Marker Expression During in Vitro Mesenchymal Stem Cell Chondrogenesis Under Hypertonic Conditions. Cellular and Molecular Biology, 6(3), 56-61.
Chicago	Ahmadyan S, Kabiri M, Tasharofi N, Hosseinzadeh S, Kehtari M, Zadeh AH, Soleimani M, Farazmand A, Hanaeeahvaz H. "The Osmolyte Type Affects Cartilage Associated Pathologic Marker Expression During in Vitro Mesenchymal Stem Cell Chondrogenesis Under Hypertonic Conditions." Cellular and Molecular Biology 6, no. 3 (2018): 56-61.
Harvard	Ahmadyan S et al. (2018) 'The Osmolyte Type Affects Cartilage Associated Pathologic Marker Expression During in Vitro Mesenchymal Stem Cell Chondrogenesis Under Hypertonic Conditions', Cellular and Molecular Biology, 6(3), pp. 56-61.
Vancouver	Ahmadyan S, Kabiri M, Tasharofi N, Hosseinzadeh S, Kehtari M, Zadeh AH, et al.. The Osmolyte Type Affects Cartilage Associated Pathologic Marker Expression During in Vitro Mesenchymal Stem Cell Chondrogenesis Under Hypertonic Conditions. Cellular and Molecular Biology. 2018;6(3):56-61.
BibTex	@article{ author = {Ahmadyan S and Kabiri M and Tasharofi N and Hosseinzadeh S and Kehtari M and Zadeh AH and Soleimani M and Farazmand A and Hanaeeahvaz H}, title = {The Osmolyte Type Affects Cartilage Associated Pathologic Marker Expression During in Vitro Mesenchymal Stem Cell Chondrogenesis Under Hypertonic Conditions}, journal = {Cellular and Molecular Biology}, volume = {6}, number = {3}, pages = {56-61}, year = {2018} }
RIS	TY - JOUR AU - Ahmadyan S AU - Kabiri M AU - Tasharofi N AU - Hosseinzadeh S AU - Kehtari M AU - Zadeh AH AU - Soleimani M AU - Farazmand A AU - Hanaeeahvaz H TI - The Osmolyte Type Affects Cartilage Associated Pathologic Marker Expression During in Vitro Mesenchymal Stem Cell Chondrogenesis Under Hypertonic Conditions JO - Cellular and Molecular Biology VL - 6 IS - 3 SP - 56 EP - 61 PY - 2018 ER -

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