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Comparison of P27 Gene Expression of Promastigote and Amastigote Forms of Leishmania Major (Mrho/Ir/75/Er) by Real-Time Rt-Pcr



Elikaee S1 ; Mohebali M1, 2 ; Eslami H3 ; Rezaei S1 ; Najafian HR1 ; Kazemirad E4 ; Keshavarz H1 ; Eshraghian MR5 ; Hajjaran H1 ; Oshaghi MA6 ; Ayazian Mavi S1
Authors
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Authors Affiliations
  1. 1. Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Center for Endemic Parasites of Iran, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Dept. of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Dept. of Parasitology, Pasteur Institute of Iran, Tehran, Iran
  5. 5. Dept. of Biostatistics and Epidemiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Dept. of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Source: Iranian Journal of Parasitology Published:2018

Abstract

Background: Cutaneous leishmaniasis (CL) is one of the world health problems. Leishmania major is the etiological agent of zoonotic cutaneous leishmaniasis (ZCL). Promastigote and amastigote are two morphological forms of Leishmania parasites that express different proteins and p27 is an important gene encoding cytochrome c oxidase (COX) component. P27 gene expresses a 27 kDa protein that essential in ATP synthesis. This study aimed to compare p27 gene expression in promastigote and amastigote forms in Iranian strain of L. major (MRHO/IR/75/ER). Methods: This study was conducted in 2015. Clinical isolates of CL patients from north, center, west and south parts of Iran were collected and identified by PCR-RFLP. After RNA extraction of promastigotes and amastigotes and cDNA synthesis, the expression level of p27 gene was compared by real-time RT-PCR. Results: By comparison of expression level between amastigote and promastigote forms of Iranian strain of L. major, up-regulation of p27 gene (2.73 fold) was observed in amastigotes. Moreover, there was no significant difference in p27 gene expression between L. major isolates. Conclusion: p27 gene and protein can be considered as a target in recombinant vaccine production and treatment process. © 2018, Tehran University of Medical Sciences (TUMS). All rights reserved.
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